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Titolo:
Characteristics of circulating growth hormone in women after acute heavy resistance exercise
Autore:
Hymer, WC; Kraemer, WJ; Nindl, BC; Marx, JO; Benson, DE; Welsch, JR; Mazzetti, SC; Volek, JS; Deaver, DR;
Indirizzi:
Univ Connecticut, Human Performance Lab, Dept Kinesiol, Storrs, CT 06269 USA Univ Connecticut Storrs CT USA 06269 Dept Kinesiol, Storrs, CT 06269 USA Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA PennState Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA Penn State Univ, Dept Kinesiol, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA Penn State Univ, Dept Dairy & Anim Sci, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 i, University Pk, PA 16802 USA Penn State Univ, Intercoll Program Physiol, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 4, volume: 281, anno: 2001,
pagine: E878 - E887
SICI:
0193-1849(200110)281:4<E878:COCGHI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; RESPONSES; DISORDERS; PROTOCOLS; RECEPTOR; ASSAYS; MEN; GH;
Keywords:
growth hormone isoforms; strength training; women; immunofunctional assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kraemer, WJ Univ Connecticut, Human Performance Lab, Dept Kinesiol, U-1110, Storrs, CT06269 USA Univ Connecticut U-1110 Storrs CT USA 06269 torrs, CT06269 USA
Citazione:
W.C. Hymer et al., "Characteristics of circulating growth hormone in women after acute heavy resistance exercise", AM J P-ENDO, 281(4), 2001, pp. E878-E887

Abstract

The effects of exercise on th molecular nature of secreted human growth hormone (GH) or its biological activity are not well understood. Plasma fron women (average age 23.6 yr, n = 35), drawn before and after an acute heavy resistance exercise test, was fractionated by size exclusion chromatographyinto three size classes namely, >60 kDa (fraction A), 30-60 kDa (fraction B), and <30 kDa (fraction C), before GH assay. Concentrations of GI in these fractions, as well as in unfractioned plasma, were measured by the Nichols immunoradiometric assay, Nation Institute of Diabetes and Digestive and Kidney Disease (NIDDK) polyclonal competitive RIA, Diagnostic System Laboratory's immunofunctional assay (measures dimerization-capable species), and the rat tibial bioassay. Significantly increased circulating GH concentrations of two-to fourfold were observed when immunoassays in unfractionated plasma samples were used, but they showed no significant change with use of the rat tibial bioassay. Significant exercise-induced increases in GH were found in fractions and C but not in fraction A. Because chemical reduction the samples before GH immunoassay significantly increase GH concentrations infractions B and C (Nichols and NIDDK kits) after exercise, it is concludedthat exercise may specifically increase release of disulfide-linked hormone molecule and/or fragments. Finally, because most of the GH release after exercise was able to dimerize the GH receptor in vitro, it is also concluded that these forms have the two intact binding sites required to initiate signal transduction in target cells.

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Documento generato il 26/09/20 alle ore 08:20:40