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Titolo:
Evidence of separate pathways for lactate uptake and release by the perfused rat heart
Autore:
Chatham, JC; Des Rosiers, C; Forder, JR;
Indirizzi:
Univ Alabama, Ctr NMR Res & Dev, Dept Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 , Dept Med, Birmingham, AL 35294 USA Johns Hopkins Univ, Sch Med, Dept Radiol, Div Magnet Resonance Res, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 ce Res, Baltimore, MD 21205 USA Univ Montreal, Dept Nutr, Montreal, PQ H2L 4M1, Canada Univ Montreal Montreal PQ Canada H2L 4M1 tr, Montreal, PQ H2L 4M1, Canada Univ Alabama, Dept Biomed Engn, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 iomed Engn, Birmingham, AL 35294 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 4, volume: 281, anno: 2001,
pagine: E794 - E802
SICI:
0193-1849(200110)281:4<E794:EOSPFL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
METABOLIC COMPARTMENTATION; SUBSTRATE UTILIZATION; OXIDATIVE-METABOLISM; GLUCOSE-OXIDATION; MYOCARDIAL FLOW; CONSCIOUS DOG; IN-VIVO; PYRUVATE; ACID; GLYCOLYSIS;
Keywords:
nuclear magnetic resonance spectroscopy; gas chromatography-mass spectrometry; diabetes; palmitate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Chatham, JC Univ Alabama, Ctr NMR Res & Dev, Dept Med, CNIR Bldg,828 8th Court S, Birmingham, AL 35294 USA Univ Alabama CNIR Bldg,828 8th Court S Birmingham AL USA 35294
Citazione:
J.C. Chatham et al., "Evidence of separate pathways for lactate uptake and release by the perfused rat heart", AM J P-ENDO, 281(4), 2001, pp. E794-E802

Abstract

The simultaneous release and uptake of lactate by the heart has been observed both in vivo and ex. vivo; however, the pathways underlying these observations have not been satisfactorily explained. Consequently, the purpose of this study was to test the hypothesis that hearts release lactate from glycolysis while simultaneously taking up exogenous lactate. Therefore, we determined the effects of fatty acids and diabetes on the regulation of lactate uptake and release. Hearts from control and 1-wk diabetic animals were perfused with 5 mM glucose, 0.5 mM [3-C-13]lactate, and 0, 0.1, 0.32, or 1.0mM palmitate. Parameters measured include perfusate lactate concentrations, fractional enrichment, and coronary flow rates, which enabled the simultaneous, but independent, measurements of the rates of 1) uptake of exogenous[C-13]lactate and 2) efflux of unlabeled lactate from metabolism of glucose. Although the rates of lactate uptake and efflux were both, similarly inhibited by the addition of palmitate, (i.e., the ratio of lactate uptake to efflux remained constant), the ratio of lactate uptake to efflux was significantly higher in the controls compared with the diabetic group (1.00 +/- 0.14 vs. 0.50 +/- 0.07, P < 0.002). These data, combined with heterogeneous C-13 enrichment of tissue lactate, pyruvate, and alanine, suggest that glycolytically derived lactate production and oxidation of exogenous lactate operate as functionally separate metabolic pathways. These results are consistent with the concept of an intracellular lactate shuttle.

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Documento generato il 23/11/20 alle ore 21:34:51