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Titolo:
Increased expression of a nucleolar Nop5/Sik family member in metastatic melanoma cells - Evidence for its role in nucleolar sizing and function
Autore:
Nakamoto, K; Ito, A; Watabe, K; Koma, Y; Asada, H; Yoshikawa, K; Shinomura, Y; Matsuzawa, Y; Nojima, H; Kitamura, Y;
Indirizzi:
Osaka Univ, Sch Med, Dept Pathol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Pathol, Suita, Osaka 5650871, Japan Osaka Univ, Sch Med, Dept Dermatol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ermatol, Suita, Osaka 5650871, Japan Osaka Univ, Sch Med, Dept Internal Med & Mol Sci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Mol Sci, Suita, Osaka 5650871, Japan Osaka Univ, Dept Mol Genet, Microbial Dis Res Inst, Suita, Osaka, Japan Osaka Univ Suita Osaka Japan Microbial Dis Res Inst, Suita, Osaka, Japan
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 4, volume: 159, anno: 2001,
pagine: 1363 - 1374
SICI:
0002-9440(200110)159:4<1363:IEOANN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE TUMOR-CELLS; MALIGNANT-MELANOMA; CANCER METASTASIS; B-16 MELANOMA; RIBOSOME BIOGENESIS; GLUCOSE STARVATION; SELECTION; COMPONENT; PROTEINS; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Ito, A Osaka Univ, Sch Med, Dept Pathol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 a 5650871, Japan
Citazione:
K. Nakamoto et al., "Increased expression of a nucleolar Nop5/Sik family member in metastatic melanoma cells - Evidence for its role in nucleolar sizing and function", AM J PATH, 159(4), 2001, pp. 1363-1374

Abstract

F10 and BL6 cells of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells can metastasize to lungs after subcutaneous injection. Differences in gene expression between the two cell lines were examined, and a greater expression of the Sik-similar protein (Sik-SP) gene was found in BL6 cells. Structurally, Sik-SP belongs to the nucleolar Nop5/Sik family whose members play central roles in ribosome biogenesis; however, the function of Sik-SP has not been examined. Cytology with green fluorescent protein-fused proteins showed that Sik-SP was localized to the nucleolus. To examine whether Sik-SP is involved in ribosome biogenesis, two parameters were measured: magnitude of ribosomal RNA synthesis per nucleus and magnitude of protein production from the same amount of mRNA of an exogenous luciferase gene. Both values and, in addition, nucleolar size were larger in COS-7 monkey kidney cells overexpressing Sik-SP and BL6 cells than in mock-transfected COS-7 and F10 cells, respectively. Sik-SP seemed to promote ribosome biogenesis in the nucleolus. Furthermore, the expression of Sik-SP seemed to confer a greater cell growth response to serum, because sucha response was greater in BL6 cells and F10 cells overexpressing: Sik-SP than in untreated and mock-transfected F10 cells. Sik-SP may render melanomacells more competent to survive through augmenting the activity of nucleolus.

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Documento generato il 23/09/20 alle ore 16:10:25