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Titolo:
Chromatin compaction and tumor cell radio sensitivity at 2 gray
Autore:
Chapman, JD; Stobbe, CC; Matsumoto, Y;
Indirizzi:
Fox Chase Canc Ctr, Dept Radiat Oncol, Philadelphia, PA 19111 USA Fox Chase Canc Ctr Philadelphia PA USA 19111 , Philadelphia, PA 19111 USA
Titolo Testata:
AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS
fascicolo: 5, volume: 24, anno: 2001,
pagine: 509 - 515
SICI:
0277-3732(200110)24:5<509:CCATCR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHINESE-HAMSTER CELLS; PREDICTS RADIATION RESPONSE; CHROMOSOME CONDENSATION; OKADAIC ACID; INTRINSIC RADIOSENSITIVITY; OXYGENATION PREDICTS; PROTEIN PHOSPHATASE; CALYCULIN-A; H3 PHOSPHORYLATION; TOPOISOMERASE-II;
Keywords:
intrinsic radiosensitivity; condensed chromatin; digital imaging; histone phosphorylation; predictive assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Chapman, JD Fox Chase Canc Ctr, Dept Radiat Oncol, 7701 Burholme Ave, Philadelphia, PA19111 USA Fox Chase Canc Ctr 7701 Burholme Ave Philadelphia PA USA 19111
Citazione:
J.D. Chapman et al., "Chromatin compaction and tumor cell radio sensitivity at 2 gray", AM J CL ONC, 24(5), 2001, pp. 509-515

Abstract

Mammalian cells at mitosis, differentiated lymphocytes, and some radiation-hypersensitive mutants in interphase contain all or a measurable portion of their chromatin in condensed/ compacted form and are hypersensitive to ionizing radiation by the mechanism described by single-hit inactivation kinetics (a). These observations led to the investigation as to whether compacted chromatin in interphase is the target that determines the widely variable a-parameters and surviving fractions of 2 Gy (SF2Gy) measured for human tumor cell lines. Six cell lines whose SF2(Gy) ranged from 0.29 to 0.73 wereused for this study. Their different radiosensitivities were associated mainly with differences in their single-hit inactivation parameters (a). Electron microscope images of interphase nuclei were optically scanned, and thepixel densities were digitized for quantitative analyses. A significant correlation between the percentage of nuclear pixels with densities similar to those found in mitotic chromosomes (percent compacted chromatin) and the alpha -inactivation parameters was observed. Digital analyses of electron and/or confocal microscope images of chromatin in interphase tumor cells in biopsy specimens could become a rapid assay for predicting the intrinsic radiosensitivity of tumor clonogens. This research has also identified some inhibitors of protein (historic) phosphatases that promote chromatin compaction and radiosensitize cells to 2-Gy dose fractions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:55:59