Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cellular proliferation and leukocyte infiltration in the rabbit cornea after photorefractive keratectomy
Autore:
Gan, L; Hamberg-Nystrom, H; Fagerholm, P; Van Setten, G;
Indirizzi:
Linkoping Univ Hosp, Dept Ophthalmol, SE-58185 Linkoping, Sweden LinkopingUniv Hosp Linkoping Sweden SE-58185 SE-58185 Linkoping, Sweden Karolinska Inst, St Eriks Eye Hosp, Stockholm, Sweden Karolinska Inst Stockholm Sweden , St Eriks Eye Hosp, Stockholm, Sweden
Titolo Testata:
ACTA OPHTHALMOLOGICA SCANDINAVICA
fascicolo: 5, volume: 79, anno: 2001,
pagine: 488 - 492
SICI:
1395-3907(200110)79:5<488:CPALII>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN; LOCALIZATION; FIBRONECTIN; APOPTOSIS; TENASCIN; SURGERY;
Keywords:
rabbits; cornea; wound healing; cell proliferation; photorefractive keratectomy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Gan, L Linkoping Univ Hosp, Dept Ophthalmol, SE-58185 Linkoping, Sweden Linkoping Univ Hosp Linkoping Sweden SE-58185 5 Linkoping, Sweden
Citazione:
L. Gan et al., "Cellular proliferation and leukocyte infiltration in the rabbit cornea after photorefractive keratectomy", ACT OPHTH S, 79(5), 2001, pp. 488-492

Abstract

Purpose: To map the proliferative activity of corneal cells during wound healing following photorefractive keratectomy (PRK) and to compare two markers for proliferation. Methods. PRK, 5- nun in diameter with a -6 D setting, was performed in oneeye of 28 New Zealand White Rabbits. The rabbits were sacrificed at time points between 12 hours and three months after surgery. The treated and fellow corneas were fixed in 10% formaldehyde, paraffin embedded, and immunohistochemically stained for proliferate cell nuclear antigen (PCNA) and at onetime point, I week, also for Ki-67. Results: Following initial sliding of the epithelial cells, the proliferative activity in the wound area starts in the leading edge (24 hours) and isspread towards the periphery. The proliferative activity peaks after one week and subsides during the following two weeks. Early (24 hours) proliferative activity is also seen in the limbal epithelium which peaks after threedays. The keratocytes express PCNA in the peripheral stroma 48 hours afterinjury. They then also migrate to repopulate the stroma under the wound area. The expression period lasts I week and subsides the following week. Leukocytes are found in the wound as early as 12 hours after injury. The cellsdisappear around the time of epithelial wound closure, i.e. after 3 days. The two proliferative markers PCNA and KI 67 show a similar distribution after surgery. Conclusion: Epithelial proliferative activity starts earlier after injury,and is preceded by leukocyte presence in the wound. The PCNA expression starts later in the keratocytes but lasts somewhat longer (3 weeks). PCNA expression appears more efficient than Ki-67 to show proliferative activity ofslow cycling cells in the cornea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 08:48:05