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Titolo:
LIPID-COMPOSITION AND PHOSPHOLIPID ASYMMETRY OF MEMBRANES FROM A SCHWANN-CELL LINE
Autore:
CALDERON RO; DEVRIES GH;
Indirizzi:
EDWARD HINES JR VET ADM HOSP,RES SERV 151,5TH AVE & ROOSEVELT RD HINES IL 60141 EDWARD HINES JR VET ADM HOSP,RES SERV 151 HINES IL 60141 UNIV NACL CORDOBA,FAC CIENCIAS MED,CATEDRA HISTOL EMBRIOL & GENET 1 RA-5000 CORDOBA ARGENTINA LOYOLA UNIV,STRITCH SCH MED,DEPT CELL BIOL NEUROBIOL & ANAT MAYWOOD IL 60153
Titolo Testata:
Journal of neuroscience research
fascicolo: 3, volume: 49, anno: 1997,
pagine: 372 - 380
SICI:
0360-4012(1997)49:3<372:LAPAOM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPPORTED PLANAR MEMBRANES; AIR-WATER-INTERFACE; ERYTHROCYTE-MEMBRANE; SURFACE EXPOSURE; AXOLEMMA; MYELIN; FIBROBLASTS; RECOGNITION; FUSION;
Keywords:
PLASMA MEMBRANE; TRANSBILAYER PHOSPHOLIPID DISTRIBUTION; SCHWANN CELL; PHOSPHOLIPID ASYMMETRY; ASYMMETRY OF SCHWANN CELL MEMBRANES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
R.O. Calderon e G.H. Devries, "LIPID-COMPOSITION AND PHOSPHOLIPID ASYMMETRY OF MEMBRANES FROM A SCHWANN-CELL LINE", Journal of neuroscience research, 49(3), 1997, pp. 372-380

Abstract

We report the total lipid composition and phospholipid asymmetry of aplasma membrane preparation isolated from a Schwann cell line (NF1T) derived from a human neurofibroma, The specific activities of three plasma membrane markers (5'-nucleotidase, Na-K-ATPase, and CNPase) were 8-fold, 12-fold, and 16-fold higher, respectively, in the plasma membrane fraction compared to the specific activities found in the total homogenate, The specific activities of the marker enzymes of intracellular membranes in the isolated plasma membrane fraction indicated littlecontamination with intracellular organelles, The enrichment of cholesterol (3-fold), sphingomyelin (3-fold), and glycolipids (cerebrosides 8-fold, sulfatides 5-fold) also indicated a high degree of purity of the plasma membrane fraction, The high content of phosphatidylinositol and phosphatidylcholine (10% and 44% of total phospholipid) and the low phosphatidyl-serine and phosphatidylethanolamine content (3% and 14%of the total phospholipid) were also characteristic of the plasma membrane fraction derived from this cell line, The transbilayer phospholipid distribution of the plasma membrane in intact cells and in the isolated plasma membrane fraction was investigated by using phospholipaseA(2) (bee venom) and sphingomyelinase (S, aureus). The phospholipid asymmetry of NF1T plasma membrane followed the general features of phospholipid asymmetry in eukaryotic cells: sphingomyelin and phosphatidylcholine were preferentially located in the outer leaflet (90% and 89%,respectively) while the aminophospholipids phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol were in the inner half ofthe membrane (85%, 96%, and 69%, respectively). A high percentage of the total plasma membrane phosphatidylinositol (31%) was found in the enter side of the membrane indicating a decreased asymmetric distribution for this negatively charged phospholipid, The phospholipid asymmetry found in the plasma membrane vesicle fraction corroborated the phospholipid asymmetry of the intact cells, thus confirming that the plasma membrane vesicles maintained the original orientation and lipid asymmetry after homogenization and/or sonication. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:33:51