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Titolo:
Isolation and study of KILSM4, a Kluyveromyces lactis gene homologous to the essential gene LSM4 of Saccharomyces cerevisiae
Autore:
Mazzoni, C; Falcone, C;
Indirizzi:
Univ Roma La Sapienza, Dept Cell & Dev Biol, Inst Pasteur, Cenci Bolognetti Fdn, I-00185 Rome, Italy Univ Roma La Sapienza Rome Italy I-00185 gnetti Fdn, I-00185 Rome, Italy
Titolo Testata:
YEAST
fascicolo: 13, volume: 18, anno: 2001,
pagine: 1249 - 1256
SICI:
0749-503X(20010930)18:13<1249:IASOKA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SM-LIKE PROTEINS; HIGH-LEVEL SECRETION; U6 SNRNA; YEAST; HOST; IDENTIFICATION; EXPRESSION; TRANSPORT; VECTORS; GROWTH;
Keywords:
Kluyveromyces lactis; Sm-like protein; Saccharomyces cerevisiae; complementation; stationary phase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Mazzoni, C Univ Roma La Sapienza, Dept Cell & Dev Biol, Inst Pasteur, Cenci Bolognetti Fdn, Piazzale Aldo Moro 5, I-00185 Rome, Italy Univ Roma La Sapienza Piazzale Aldo Moro 5 Rome Italy I-00185
Citazione:
C. Mazzoni e C. Falcone, "Isolation and study of KILSM4, a Kluyveromyces lactis gene homologous to the essential gene LSM4 of Saccharomyces cerevisiae", YEAST, 18(13), 2001, pp. 1249-1256

Abstract

We have isolated the KILSM4 gene as a multicopy suppressor of a Kluyveromyves lactis mutant which shows a rag phenotype (resistance to antimycin A onglucose). This gene is homologous to the ScLSM4 of Saccharomyces cerevisiae, which codes for an essential 187 amino acid protein containing Sm-like domains. These motifs are present in the evolutionarily conserved family of the Sm-like proteins, which are involved in a large number of cellular processes, including pre-mRNA splicing and mRNA decapping. We demonstrated thatthe first 72 amino acids of KILsm4p, which contain the Sm-like domains, can restore cell viability in both K. lactis and S. cerevisiae cells lacking the wild-type protein. However, the absence of the carboxy-terminal region resulted in a remarkable loss of cell viability in the stationary phase. The KILSM4 sequence has been deposited in the EMBL Data library under Accession No. AJ311719. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:17:07