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Titolo:
Elevated endosomal pH in HeLa cells overexpressing mutant dynamin can affect infection by pH-sensitive viruses
Autore:
Huber, M; Brabec, M; Bayer, N; Blaas, D; Fuchs, R;
Indirizzi:
Univ Vienna, Dept Pathophysiol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 t Pathophysiol, A-1090 Vienna, Austria Univ Vienna, Div Biochem, Inst Med Biochem VBC, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 ed Biochem VBC, A-1030 Vienna, Austria
Titolo Testata:
TRAFFIC
fascicolo: 10, volume: 2, anno: 2001,
pagine: 727 - 736
SICI:
1398-9219(200110)2:10<727:EEPIHC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COATED VESICLE FORMATION; HUMAN RHINOVIRUS SEROTYPE-2; TRANS-GOLGI NETWORK; LIPOPROTEIN RECEPTOR; ENDOCYTIC PATHWAY; PLASMA-MEMBRANE; IN-VITRO; ENTRY; PROTEIN; VACUOLAR;
Keywords:
dynamin(K44A); endocytosis; endosomal pH; HeLa cells; HRV2; minor receptor group human rhinovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, R Univ Vienna, Dept Pathophysiol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 Austria
Citazione:
M. Huber et al., "Elevated endosomal pH in HeLa cells overexpressing mutant dynamin can affect infection by pH-sensitive viruses", TRAFFIC, 2(10), 2001, pp. 727-736

Abstract

Many viruses gain access to the cell via the endosomal route and require low endosomal pH for infectivity. The GTPase dynamin is essential for clathrin-dependent endocytosis, and in HeLa cells overexpressing the nonfunctional dynamin(K44A) mutant the formation of clathrin-coated vesicles is halted. HRV2, a human minor group rhinovirus, is internalized by members of the low-density lipoprotein receptor family in a clathrin-independent manner. Thelow endosomal pH then leads to conversion of the capsid to C-antigen, which is required for release (uncoating) and transfer of the viral RNA into the cytosol and de novo synthesis of infectious virus. We here demonstrate that overexpression of dynamin(K44A) reduces this antigenic conversion and results in diminished viral synthesis. In contrast, lysosomal degradation is unaffected. The kinetics of the formation of C-antigen in vitro and in vivosuggest that the pH in endosomes is elevated by about 0.4 units upon overexpression of dynamin(K44A). As a consequence, HRV2 uncoating is diminished early after internalization but attains control levels upon prolonged internalization. Thus, overexpression of dynamin(K44A), in addition to trafficking defects, results in an elevated endosomal pH and thereby affects virus infection and most likely endosomal sorting and processing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:25:53