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Titolo:
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on corticotrophin-releasing hormone, arginine vasopressin, and pro-opiomelanocortin mRNA levels in the hypothalamus of the cynomolgus monkey
Autore:
Shridhar, S; Farley, A; Reid, RL; Foster, WG; Van Vugt, DA;
Indirizzi:
Queens Univ, Dept Obstet & Gynecol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 ecol, Kingston, ON K7L 3N6, Canada Queens Univ, Dept Physiol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 siol, Kingston, ON K7L 3N6, Canada Hlth Canada, Hlth Protect Branch, Environm & Occupat Toxicol Div, Ottawa, ON K1A 0L2, Canada Hlth Canada Ottawa ON Canada K1A 0L2 icol Div, Ottawa, ON K1A 0L2, Canada
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 2, volume: 63, anno: 2001,
pagine: 181 - 188
SICI:
1096-6080(200110)63:2<181:TEO2OC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
OVARIECTOMIZED RHESUS-MONKEY; RESISTANT RAT STRAIN; LUTEINIZING-HORMONE; MACACA-MULATTA; TISSUE DISTRIBUTION; CALLITHRIX-JACCHUS; IMMUNE-SYSTEM; AH-RECEPTOR; IN-VITRO; TCDD;
Keywords:
2,3,7,8-tetrachlorodibenzo-p-dioxin; cynomolgus monkeys; hypothalamic-pituitary-adrenal axis; corticotropin-releasing hormone; arginine vasopressin; pro-opiomelanocortin; mRNA; in situ hybridization histochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Van Vugt, DA Queens Univ, Dept Obstet & Gynecol, 3022 Etherington Hall, Kingston, ON K7L 3N6, Canada Queens Univ 3022 Etherington Hall Kingston ON Canada K7L 3N6
Citazione:
S. Shridhar et al., "The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on corticotrophin-releasing hormone, arginine vasopressin, and pro-opiomelanocortin mRNA levels in the hypothalamus of the cynomolgus monkey", TOXICOL SCI, 63(2), 2001, pp. 181-188

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a widespread environmental contaminant that has profound deleterious effects on development and reproduction. TCDD may act at one or more levels to alter the hypothalamic-pituitary-adrenal (HPA) and hypothalamic-pituitary-gonadal (HPG) axes. The objective of this study was to investigate whether TCDD modulates neuroendocrine systems by altering gene expression of arginine vasopressin (AVP), corticotrophin-releasing hormone (CRH), or pro-opiomelanocortin (POMC), which are important neuroregulators of the HPA and HPG axes. Four groups of female cynomolgus monkeys (Macaca fascicularis) were administered daily oral doses of gelatin capsule containing TCDD (0, 1, 5, or 25 ng/kg body weight) mixed with glucose 5 days a week for 1 year. At the end of the dosing period, animals were euthanized and brains were harvested. CRH, AVP, and POMC mRNA levels were semiquantified by in situ hybridization histochemistry on 30-mum coronal sections of the brain. Blood collected on the day of euthanasia was assayed for cortisol and progesterone. CRH mRNA levels in the paraventricularnucleus (PVN) were significantly increased by the 2 higher TCDD doses (5 and 25 ng/kg/day) compared to controls (p < 0.05). There was a trend towardsincreased AVP mRNA levels in both the supraoptic nucleus (SON) and PVN. Noeffect of TCDD on POMC was observed. Cortisol levels were significantly increased in TCDD-exposed animals. Progesterone concentrations and menstruation data indicated that TCDD did not interfere with ovulation. We conclude that TCDD stimulated the HPA axis by a central effect involving CRH, but hadno effect on the HPG axis at the doses tested.

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Documento generato il 25/01/20 alle ore 07:04:30