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Titolo:
Effects of beta-estradiol and bisphenol a on heat shock protein levels andlocalization in the mouse uterus are antagonized by the antiestrogen ICI 182,780
Autore:
Papaconstantinou, AD; Fisher, BR; Umbreit, TH; Goering, PL; Lappas, NT; Brown, KM;
Indirizzi:
George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA George Washington Univ Washington DC USA 20052 , Washington, DC 20052 USA US FDA, Ctr Devices & Radiol Hlth, Rockville, MD 20857 USA US FDA Rockville MD USA 20857 ices & Radiol Hlth, Rockville, MD 20857 USA George Washington Univ, Dept Forens Sci, Washington, DC 20052 USA George Washington Univ Washington DC USA 20052 , Washington, DC 20052 USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 2, volume: 63, anno: 2001,
pagine: 173 - 180
SICI:
1096-6080(200110)63:2<173:EOBABA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN-RECEPTOR-ALPHA; MESSENGER-RIBONUCLEIC-ACID; UTEROTROPHIC ACTIVITY; OVARIECTOMIZED RATS; HUMAN ENDOMETRIUM; GENE-EXPRESSION; 90 KDA; C-FOS; GROWTH; HSP90;
Keywords:
bisphenol A (BPA); ICI 182; 780 (ICI); beta-estradiol (E-2); estrogen receptor (ER); uterus; heat shock proteins; hsp90 alpha localization; B6C3F1 mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, KM George Washington Univ, Dept Biol Sci, 332 Lisner Hall,2023 G StNW, Washington, DC 20052 USA George Washington Univ 332 Lisner Hall,2023 GSt NW Washington DC USA 20052
Citazione:
A.D. Papaconstantinou et al., "Effects of beta-estradiol and bisphenol a on heat shock protein levels andlocalization in the mouse uterus are antagonized by the antiestrogen ICI 182,780", TOXICOL SCI, 63(2), 2001, pp. 173-180

Abstract

Bisphenol A (BPA) exhibits many estrogen-like effects in the rodent uterus, but not all of these can be attenuated by antiestrogens. This suggests the involvement of alternate pathways of BPA action that do not involve the estrogen receptor (ER). An examination of the in vivo effects of BPA on uterine gene expression and protein levels should contribute to an understanding of its mechanism of action. In this study we examined the dose-related effects of BPA on levels of a suite of heat shock proteins (hsps) and on the localization of hsp90 alpha, a chaperone of the ER, in uteri of ovariectomized B6C3F1 mice and compared these effects with those of beta -estradiol (E-2). The antiestrogen ICI 182,780 (ICI) was co-administered with BPA or E-2in order to examine the potential role of the ER. BPA, although less potent than E-2, increased hsp90 alpha and grp94 to similar levels, but was muchless effective than E-2 in increasing levels of hsp72. Treatment with 100 mg BPA/kg/day or 2 mug E-2/kg/day increased hsp90 alpha to 300% of control levels and altered its tissue expression pattern. In uteri of corn oil (control)-treated mice, hsp90 alpha predominantly localized in the cytoplasm and nuclei of epithelial cells. Upon treatment with BPA or E-2 there was increased intensity of staining in the stroma and myometrium, and in the epithelium hsp90 alpha was localized almost exclusively in the cytoplasm. The effects of BPA or E-2 on hsp levels and hsp90 alpha localization were attenuated by ICI. These results suggest an involvement of the ER in BPA- and E-2-induced increases in uterine levels of hsp90 alpha, grp94, and hsp72, and localization of hsp90 alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:48:26