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Titolo:
Acrosin activity in turkey spermatozoa assay by clinical method and effectof zinc and benzamidine on the activity
Autore:
Glogowski, J; Jankowski, J; Faruga, A; Ottobre, JS; Ciereszko, A;
Indirizzi:
Polish Acad Sci, Inst Anim Reprod & Food Res, Semen Biol Grp, PL-10747 Olsztyn, Poland Polish Acad Sci Olsztyn Poland PL-10747 ol Grp, PL-10747 Olsztyn, Poland Warmia & Masuria Univ, Dept Poultry Sci, Olsztyn, Poland Warmia & Masuria Univ Olsztyn Poland Dept Poultry Sci, Olsztyn, Poland Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 pt Anim Sci, Columbus, OH 43210 USA
Titolo Testata:
THERIOGENOLOGY
fascicolo: 5, volume: 56, anno: 2001,
pagine: 889 - 901
SICI:
0093-691X(20010915)56:5<889:AAITSA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM ACROSIN; VITELLINE MEMBRANE; FERTILIZATION; CHICKEN; STORAGE; HENS; PENETRATION; HYDROLYSIS; INVITRO; ENZYMES;
Keywords:
turkey; spermatozoa; seminal plasma; acrosin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ciereszko, A Polish Acad Sci, Inst Anim Reprod & Food Res, Semen Biol Grp,POB 55,Ul Tuwima 10, PL-10747 Olsztyn, Poland Polish Acad Sci POB 55,Ul Tuwima 10 Olsztyn Poland PL-10747
Citazione:
J. Glogowski et al., "Acrosin activity in turkey spermatozoa assay by clinical method and effectof zinc and benzamidine on the activity", THERIOGENOL, 56(5), 2001, pp. 889-901

Abstract

We optimized a clinical assay developed for measuring total acrosin activity for mammalian and fish semen for use in turkey spermatozoa. The main modifications included dilution of semen to a final concentration of 25 to 1000 x 10(3) spermatozoa, an increase of Triton X-100 concentration to 0.05% and 1 hr preincubation without substrate. Acrosin activity in turkey spermatozoa was much higher than in human spermatozoa (about 100-times) but similar to that of boar sperm, To optimize this assay for turkey spermatozoa, it was necessary to use higher Triton X-100 concentrations in the reaction mixture. There was a better catalytic efficiency at higher temperatures and a special requirement for a preincubation period for proacrosin activation. We observed high inhibition of acrosin activity by zinc added during preincubation (90% at 0.01 mM of zinc chloride). Benzamidine also inhibited turkeyacrosin, and the extent of inhibition was similar for the incubation or preincubation period. When zinc ions were added during incubation, this inhibition was lower (24%). The results suggest that zinc influences proacrosin activation of turkey spermatozoa. This influence may be important for successful long-term storage of spermatozoa in the hen's oviduct. (C) 2001 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 21:53:43