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Titolo:
The CCK-2 receptor is located on the ECL cell, but not on the parietal cell
Autore:
Bakke, I; Qvigstad, G; Sandvik, AK; Waldum, HL;
Indirizzi:
Norwegian Univ Sci & Technol, Fac Med, Dept Physiol & Biomed Engn, NO-7489Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway NO-7489 9Trondheim, Norway Norwegian Univ Sci & Technol, Fac Med, Dept Intraabdominal Dis, NO-7489 Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway NO-7489 Trondheim, Norway
Titolo Testata:
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
fascicolo: 11, volume: 36, anno: 2001,
pagine: 1128 - 1133
SICI:
0036-5521(200111)36:11<1128:TCRILO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROCHROMAFFIN-LIKE CELLS; GASTRIC-ACID SECRETION; PERFUSED RAT STOMACH; GUINEA-PIG STOMACH; IMMUNOHISTOCHEMICAL LOCALIZATION; HISTAMINE-RELEASE; EXPRESSION; DOG;
Keywords:
corpus mucosa; fluo-peptide; histamine release; regulation of gastric acid secretion; tyramide signal amplification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Bakke, I Norwegian Univ Sci & Technol, Fac Med, Dept Physiol & Biomed Engn, NO-7489Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway NO-7489 m, Norway
Citazione:
I. Bakke et al., "The CCK-2 receptor is located on the ECL cell, but not on the parietal cell", SC J GASTR, 36(11), 2001, pp. 1128-1133

Abstract

The interrelationship between histamine and gastrin in the physiological regulation of gastric acid secretion is still a matter of dispute. CCK-2 receptors are located on enterochromaffin-like (ECL) cells in corpus mucosa and gastrin stimulates acid production by releasing histamine from the ECL cells, which in turn stimulates the parietal cells. Whether parietal cells also possess gastrin receptors of physiological significance is unclear. The aim of the present study was to localize the CCK-2 receptor cellularly and concomitantly demonstrate a gastrin receptor response (histamine release). Methods: Fluorescein labelled cholecystokinin-8 (Fluo-CCK-8) was added to the arterial infusion to totally isolated, vascularly perfused rat stomachs to a final concentration of 130 pmol L-1 for I min. either alone or along with 520 nmol(.1) CCK-8 after 10-min pre-perfusion with CCK-8. Immediately after the Fluo-CCK-8 had reached the oxyntic mucosa, biopsies were taken andthe binding sites were localized by double immunohistochemistry combined with the tyramide signal amplification (TSA) technique. Venous histamine wasmeasured before and during stimulation. Results: Fluo-CCK-8 (130 pM) evoked histamine release. and binding sites were found in the basal part of corpus mucosa, co-localized with histidine decarbocylase (HDC) immunoreactive ECL cells. No binding of Fluo-CCK was found in the mid-glandular region of corpus, dominated by parietal cells. Binding of Fluo-CCK-8 was abolished by concomitant perfusion with excess CCK-8. Conclusion: Fluo-CCK-8 given to isolated rat stomachs in a physiological concentration binds to CCK-2 receptors on ECL cells and causes histamine release. whereas no binding of Fluo-CCK-8 to parietal cells was found.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:59:11