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Titolo:
Activation of pig oocytes using calcium ionophore: Effect of the protein kinase inhibitor 6-dimethyl aminopurine
Autore:
Jilek, F; Huttelova, R; Petr, J; Holubova, M; Rozinek, J;
Indirizzi:
Czech Univ Agr, Dept Vet Sci, Prague 46521, Czech Republic Czech Univ AgrPrague Czech Republic 46521 Prague 46521, Czech Republic Pronatal Sanatorium, IVF Unit, Prague, Czech Republic Pronatal SanatoriumPrague Czech Republic Unit, Prague, Czech Republic Res Inst Anim Prod, Dept Reprod Biol, Prague, Czech Republic Res Inst AnimProd Prague Czech Republic d Biol, Prague, Czech Republic
Titolo Testata:
REPRODUCTION IN DOMESTIC ANIMALS
fascicolo: 3-4, volume: 36, anno: 2001,
pagine: 139 - 145
SICI:
0936-6768(200108)36:3-4<139:AOPOUC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; IN-VITRO FERTILIZATION; CELL-CYCLE; 6-DIMETHYLAMINOPURINE 6-DMAP; PARTHENOGENETIC ACTIVATION; BOVINE OOCYTES; PORCINE OOCYTES; MOUSE EGGS; MEIOTIC MATURATION; EMBRYO DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Jilek, F Czech Univ Agr, Dept Vet Sci, Prague 46521, Czech Republic Czech Univ Agr Prague Czech Republic 46521 6521, Czech Republic
Citazione:
F. Jilek et al., "Activation of pig oocytes using calcium ionophore: Effect of the protein kinase inhibitor 6-dimethyl aminopurine", REPROD DOM, 36(3-4), 2001, pp. 139-145

Abstract

The aim of our study was to investigate the parthenogenetic activation of in vitro matured pig oocytes after their combined treatment with calcium ionophore A 23187 and the inhibitor of protein kinases, 6-dimethylaminopurine(6-DMAP) and to study the further embryonic development of oocytes activated using this treatment. The oocytes were exposed to ionophore (10, 25 or 50 mum) for 0.5, 1, 3, 5 or 7 min and then cultured with 6-DMAP (0 or 2 mum)The highest activation rate (up to 88% of the activated eggs reached the pronuclear stage) was observed after combined treatment of the oocytes with 50 mum ionophore and 6-DMAP. The highest rate of embryonic development was observed after treatment with 25 mum ionophore without 6-DMAP, when up to 51% of the eggs developed beyond two-cell stage, 2% of the eggs developed upto the stage of morula and up to 3% of the eggs reached the stage of blastocyst. When 50 mum ionophore was used, the embryonic development of the activated eggs was arrested before the morula and blastocyst stage. After treatment of the activated eggs with 6-DMAP, we did not observe any developmentbeyond the stage of 16 blastomeres. We can conclude that combined treatment with calcium ionophore A 23187 and 6-DMAP increases the activation rate in pig oocytes matured in vitro, but this combined treatment exerts a detrimental effect on further embryonic development of the activated eggs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:57:02