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Titolo:
A new DNA extraction method for high-throughput marker analysis in a large-genome species such as Triticum aestivum
Autore:
Stein, N; Herren, G; Keller, B;
Indirizzi:
Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8008 Biol, CH-8008 Zurich, Switzerland
Titolo Testata:
PLANT BREEDING
fascicolo: 4, volume: 120, anno: 2001,
pagine: 354 - 356
SICI:
0179-9541(200108)120:4<354:ANDEMF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT DNA; PCR; PROTOCOL; AMPLIFICATION; WHEAT; RAPD;
Keywords:
Triticum aestivum; DNA extraction; high-throughput; marker-assisted selection; RFLP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Keller, B Univ Zurich, Inst Plant Biol, Zollikerstr 107, CH-8008 Zurich, Switzerland Univ Zurich Zollikerstr 107 Zurich Switzerland CH-8008 tzerland
Citazione:
N. Stein et al., "A new DNA extraction method for high-throughput marker analysis in a large-genome species such as Triticum aestivum", PLANT BREED, 120(4), 2001, pp. 354-356

Abstract

Gene mapping and marker-assisted selection in complex, polyploid genomes still relies strongly on restriction fragment length polymorphism (RFLP) analysis, as conversion of RFLP to polymerase chain reaction (PCR) markers canbe very difficult. DNA extraction in amounts suitable for RFLP analysis represents the most time consuming and labour-intensive step in molecular marker analysis of plant populations. In this paper. a new flexible method forplant DNA extraction is presented. It allows a high-throughput of samples in a short time without the need for freezing or lyophilizing the plant material. The method allows the isolation of genomic DNA with a yield of = 100mug for a minimal amount of 200 mug of leaf material. This is sufficient for work with large-genome plant species such as hexaploid wheat, where 20 mug of genomic DNA are required for a single RFLP analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:16:18