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Titolo:
Application of AFLP for the detection of sex-specific markers in hemp
Autore:
Flachowsky, H; Schumann, E; Weber, WE; Peil, A;
Indirizzi:
Univ Halle Wittenberg, Inst Plant Breeding & Plant Protect, D-06108 Halle Saale, Germany Univ Halle Wittenberg Halle Saale Germany D-06108 8 Halle Saale, Germany
Titolo Testata:
PLANT BREEDING
fascicolo: 4, volume: 120, anno: 2001,
pagine: 305 - 309
SICI:
0179-9541(200108)120:4<305:AOAFTD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPARAGUS-OFFICINALIS L; DNA; IDENTIFICATION; SILENE;
Keywords:
Cannabis sativa; AFLP; male-specific markers; sex determination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Peil, A Univ Halle Wittenberg, Inst Plant Breeding & Plant Protect, LudwigWucherer Str 2, D-06108 Halle Saale, Germany Univ Halle Wittenberg Ludwig Wucherer Str 2 Halle Saale Germany D-06108
Citazione:
H. Flachowsky et al., "Application of AFLP for the detection of sex-specific markers in hemp", PLANT BREED, 120(4), 2001, pp. 305-309

Abstract

Two dioecious hemp accessions (Can18 and Can17) were tested by bulked segregant analysis for polymorphisms between male and female bulks with amplified fragment length polymorphisms. Thirty-nine primer combinations were tested and 20 of these yielded one to three male-specific bands. In contrast, no female-specific band was detected. Eight of these primer combinations were used for testing 80 progeny plants from a cross between two plants from Can18 and 30 plants from Can17. A total of 16 and 17 male-specific fragmentswere obtained for Can18 and Can17, respectively. Eleven fragments exhibited the same fragment size in both accessions. All male plants, but not one female plant, showed the respective polymorphic band with each of the eight primer combinations. Problems regarding sex determination under field conditions were successfully overcome by testing plants that had been grown in small pots in a greenhouse. The abundant number of potential markers for themale sex, their complete cosegregation with male plants and the absence ofmarkers for the female sex support the presence of a male sex chromosome in hemp.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:57:28