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Titolo:
Thalamic stimulation largely elicits orthodromic, rather than antidromic, cortical activation in an auditory thalamocortical slice
Autore:
Rose, HJ; Metherate, R;
Indirizzi:
Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA 92697 USA Univ CalifIrvine Irvine CA USA 92697 obiol & Behav, Irvine, CA 92697 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 106, anno: 2001,
pagine: 331 - 340
SICI:
0306-4522(2001)106:2<331:TSLEOR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE BARREL CORTEX; EVOKED-POTENTIALS; HORSERADISH-PEROXIDASE; IDENTIFIED NEURONS; STRIATE CORTEX; CAT; ORGANIZATION; PROJECTIONS; RESPONSES; PATTERNS;
Keywords:
corticothalamic; brain slice; sensory cortex; in vitro; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Metherate, R Univ Calif Irvine, Dept Neurobiol & Behav, 2205 Biol Sci 2, Irvine, CA 92697 USA Univ Calif Irvine 2205 Biol Sci 2 Irvine CA USA 92697 697 USA
Citazione:
H.J. Rose e R. Metherate, "Thalamic stimulation largely elicits orthodromic, rather than antidromic, cortical activation in an auditory thalamocortical slice", NEUROSCIENC, 106(2), 2001, pp. 331-340

Abstract

Stimulation of the medial geniculate body in an auditory thalamocortical slice elicits a short-latency current sink in the middle cortical layers, aswould be expected following activation of thalamocortical relay neurons. However, corticothalamic neurons can have axon collaterals that project to the middle layers, thus, a middle-layer current sink could also result from antidromic activation of corticothalamic neurons and their axon collaterals. The likelihood of thalamic stimulation activating corticothalamic neuronswould be reduced substantially if the corticothalamic pathway was not wellpreserved in the slice, and/or if the threshold for antidromic activation was significantly higher than for orthodromic activation. To determine the prevalence and threshold of antidromic activation, we recorded intracellularly from day 14-17 mouse brain slices containing infragranular cortical neurons while stimulating the medial geniculate or thalamocortical pathway. Antidromic spikes were confirmed by spike collision and characterized according to spike latency 'jitter' and the ability to follow a high-frequency (100 Hz) stimulus train. The ability to follow a 100-Hz tetanus was a reliableindicator of antidromic activation, but both antidromic and orthodromic spikes could have low jitter. Thalamic stimulation produced antidromic activation in two of 69 infragranular cortical neurons (< 3%), indicating the presence of antidromic activity, but implying a limited corticothalamic connection in the slice. Antidromic spikes in 13 additional neurons were obtainedby stimulating axons in the thalamocortical pathway. The antidromic threshold averaged 214 +/- 40.6 muA (range 6-475 muA), over seven times the orthodromic threshold for medial geniculate-evoked responses in layer IV extracellular (28 +/- 5.4 muA) or intracellular (27 +/- 5.6 muA) recordings. We conclude that medial geniculate stimulation activates relatively few corticothalamic neurons. Conversely, low-intensity thalamic stimulation strongly activates thalamocortical neurons. Thus, at low-stimulus intensities, the auditory thalamocortical slice can be used to probe mechanisms of thalamccortical function with limited antidromic activation of corticothalamic neurons. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 13:48:49