Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
In situ class switching and differentiation to IgA-producing cells in the gut lamina propria
Autore:
Fagarasan, S; Kinoshita, K; Muramatsu, M; Ikuta, K; Honjo, T;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Med Chem, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Med Chem, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
NATURE
fascicolo: 6856, volume: 413, anno: 2001,
pagine: 639 - 643
SICI:
0028-0836(20011011)413:6856<639:ISCSAD>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTIDINE DEAMINASE AID; B-CELLS; SURFACE-RECEPTORS; MARGINAL ZONE; PLASMA-CELLS; CD40 LIGAND; FC RECEPTOR; MICE; ACTIVATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Honjo, T Kyoto Univ, Grad Sch Med, Dept Med Chem, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan Kyoto Univ Yoshida Konoe Cho Kyoto Japan 6068501 6068501, Japan
Citazione:
S. Fagarasan et al., "In situ class switching and differentiation to IgA-producing cells in the gut lamina propria", NATURE, 413(6856), 2001, pp. 639-643

Abstract

One of the front lines of the immune defence is the gut mucosa, where immunoglobulin-alpha (IgA) is continuously produced to react with commensal bacteria and dietary antigens. It is generally accepted that, after antigenic stimulation in the Peyer's patches, IgA(+) lymphoblasts (B220(+)IgA(+)) migrate through the lymph and blood circulation, and eventually home to the lamina propria of the intestine(1,2). Mice that lack activation-induced cytidine deaminase (AID) are defective in class switch recombination (CSR) and somatic hypermutation(3). CSR changes the immunoglobulin heavy chain constant region (CH) gene being expressed from C mu to other CH genes, resulting in a switch of the immunoglobulin isotype from IgM to IgG, IgE or IgA. AID(-/-) mice also secrete large amounts of immunoglobulin-m (IgM) into faeces, and accumulate B220(-)IgM(+) plasma cells as well as B220(+)IgM(+) cells inthe gut. Here we show that lamina propria B220(+)IgA(+) cells have just completed CSR, as they still express both AID and transcripts from circular DNA that has been 'looped-out' during CSR. Lamina propria IgM(+) B cells seem to be pre-committed to switching to IgA(+) in vitro as well as in vivo. Culturing lamina propria IgM(+) B cells together with lamina propria stromalcells enhances preferential switching and differentiation of B cells to IgA(+) plasma cells. We conclude that IgA(+) cells in the gut lamina propria are generated in situ from B220(+)IgM(+) lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 02:01:13