Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Antitumor activity of cationic lipid complexed with immunostimulatory DNA
Autore:
Rudginsky, S; Siders, W; Ingram, L; Marshall, J; Scheule, R; Kaplan, J;
Indirizzi:
Genzyme Corp, Framingham, MA 01701 USA Genzyme Corp Framingham MA USA 01701 nzyme Corp, Framingham, MA 01701 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 4, anno: 2001,
pagine: 347 - 355
SICI:
1525-0016(200110)4:4<347:AAOCLC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL-DNA; PLASMID DNA; INTERFERON-GAMMA; DENDRITIC CELLS; GENE-TRANSFER; CPG MOTIFS; ACTIVATION; MURINE; RESPONSES; GROWTH;
Keywords:
cationic lipid; DNA; cytokines; CpG motif; innate immunity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kaplan, J Genzyme Corp, Framingham, MA 01701 USA Genzyme Corp Framingham MA USA 01701 , Framingham, MA 01701 USA
Citazione:
S. Rudginsky et al., "Antitumor activity of cationic lipid complexed with immunostimulatory DNA", MOL THER, 4(4), 2001, pp. 347-355

Abstract

We previously reported that treatment of intraperitoneal tumors with complexes of cationic lipid and noncoding plasmid DNA leads to the development of a specific, cytotoxic T-cell response correlating with the rejection of established tumor cells as well as subsequent tumor re-challenge. Here, focusing on an intraperitoneal AB12 mesothelioma model, we show that the anticancer effects of the lipid:DNA complex are associated with DNA containing immunostimulatory CpG motifs. Complexes prepared with cationic lipid and bacteria[ plasmid DNA, Escherichia coil genomic DNA fragments, or synthetic immunostimulatory CpG oligodeoxynucleotides provided a substantial survival benefit, whereas eukaryotic DNA and methylated bacterial DNA had little or notherapeutic activity. Alternative inflammatory stimuli such as thioglycolate, poly(I:C), and incomplete or complete Freund's adjuvant failed to reproduce the antitumor activity obtained with the lipid:DNA complex. The innateimmune response triggered by lipid:DNA complexes led to the development ofa systemic immune response against tumor cells that allowed animals to reject tumors not only at the intraperitoneal treatment site, but also at a distal subcutaneous site. These data demonstrate that immunostimulatory DNA complexed with cationic lipid is a potent inducer of innate and adaptive immune responses against tumor cells and represents a potentially useful tool in the immunotherapy of cancers for which tumor-associated antigens have not been identified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 18:11:18