Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae
Autore:
Versele, M; Thevelein, JM;
Indirizzi:
Katholieke Univ Leuven, Inst Plantkunde & Microbiol, Lab Mol Celbiol, B-3001 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3001 l, B-3001 Louvain, Belgium
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 6, volume: 41, anno: 2001,
pagine: 1311 - 1326
SICI:
0950-382X(200109)41:6<1311:LACETA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOTONIC-DYSTROPHY KINASE; CAMP PATHWAY; GLUCOSE-ACTIVATION; CATALYTIC SUBUNITS; REGULATORY SUBUNIT; GENE DISRUPTION; S-CEREVISIAE; CELL-CYCLE; YEAST; RAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Thevelein, JM Katholieke Univ Leuven, Inst Plantkunde & Microbiol, Lab MolCelbiol, Kasteelpk Arenberg 31, B-3001 Louvain, Belgium Katholieke Univ Leuven Kasteelpk Arenberg 31 Louvain Belgium B-3001
Citazione:
M. Versele e J.M. Thevelein, "Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae", MOL MICROB, 41(6), 2001, pp. 1311-1326

Abstract

The addition of glucose to derepressed cells of the yeast Saccharomyces cerevisiae triggers activation of the cAMP pathway with a rapid drop in stress resistance as a consequence. We have isolated the LRE1 gene as a multicopy suppressor of glucose-induced loss of heat resistance. Overexpression of LRE1 in a wild-type strain causes the same phenotype as observed in strainswith reduced activity of the cAMP-PKA pathway: higher heat resistance and enhanced trehalose levels. Deletion of LRE1 results in the opposite phenotypes. Epistasis analysis indicates that these effects are independent of cAMP and PKA, of the protein kinases Yak1, Sch9 and Rim15 and of the transcription factors Msn2 and Msn4. Lre1 has recently been isolated in a two-hybridscreen using the conserved protein kinase Cbk1 as a bait. Cbk1 controls the expression of CTS1 (encoding chitinase) through the transcription factor Ace2. We demonstrate here that overexpression of LRE1 represses CTS1 whereas deletion of LRE1 induces the expression of CTS1. Repression of CTS1 results in deficient cell separation as a result of inefficient degradation of the chitin ring after cytokinesis. Neither deletion nor overexpression of LRE1 has any effect on CTS1 expression in a cbk1 Delta mutant, indicating that Lre1 inhibits Cbk1. In addition, we show that increased trehalose accumulation and increased heat resistance caused by overexpression of LRE1 are also the result of inhibition of Cbk1, revealing a novel control pathway for certain targets affected by PKA. The yeast genome contains a homologue of LRE1, YDR528w, which we have called HLR1 (for homologue of Lre1). Deletion and overexpression of HLR1 causes similar but less pronounced effects compared with LRE1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 20:00:03