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Titolo:
Detection of bcl-2/IgH rearrangements by quantitative-competitive PCR and capillary electrophoresis
Autore:
Telatar, M; Grody, WW; Emmanouilides, C;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Dept Pediat, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Div Hematol Oncol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
MOLECULAR DIAGNOSIS
fascicolo: 3, volume: 6, anno: 2001,
pagine: 161 - 168
SICI:
1084-8592(200109)6:3<161:DOBRBQ>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; AUTOLOGOUS BONE-MARROW; NON-HODGKINS-LYMPHOMA; FOLLICULAR LYMPHOMA; PERIPHERAL-BLOOD; TRANSPLANTATION; CELLS; T(14-18);
Keywords:
capillary electrophoresis; bcl-2; Rituximab;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Grody, WW Univ Calif Los Angeles, Sch Med, Dept Pathol & Lab Med, 10833 LeConte Ave, Los Angeles, CA 90095 USA Univ Calif Los Angeles 10833 Le ConteAve Los Angeles CA USA 90095
Citazione:
M. Telatar et al., "Detection of bcl-2/IgH rearrangements by quantitative-competitive PCR and capillary electrophoresis", MOL DIAGN, 6(3), 2001, pp. 161-168

Abstract

Background: PCR is the primary method for detecting minimal residual disease in hematologic cancers. One such gene target is the bcl-2/immunoglobulinheavy chain (IgH) translocation found in a majority of cases of follicularlymphoma. Methods and Results: We report an accurate method for quantitative detection of the bcl-2/IgH translocation marker of follicular lymphoma in a seriesof patients in various stages of remission and relapse who had been treated with a combination of ifosfamide, mitoxantrone, and etoposide (MIN-E) chemotherapy and monoclonal anti-CD20 antibody (Rituximab). The approach uses seminested PCR followed by analysis of the products on a fluorescent capillary electrophoresis system. The quantitation of bcl-2/IgH translocation-positive cells was sensitive and reproducible, capable of detecting as few as five malignant cells out of 300,000 normal cells. Conclusion: Quantitative PCR enables one to monitor the kinetics of tumor reduction in patients treated with MINE chemotherapy in combination with Rituximab.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:16:23