Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of age and food restriction on oxidative DNA damage and antioxidant enzyme activities in the mouse aorta
Autore:
Guo, ZM; Yang, H; Hamilton, ML; VanRemmen, H; Richardson, A;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Physiol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 pt Physiol, San Antonio, TX 78229 USA Univ Texas, Hlth Sci Ctr, Sam & Ann Barshop Ctr Longev & Aging Studies, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 ng Studies, San Antonio, TX 78229 USA S Texas Vet Hlth Care Syst, Ctr Geriatr Res Educ & Clin, Audie L Morphy Div, San Antonio, TX 78229 USA S Texas Vet Hlth Care Syst San Antonio TX USA78229 Antonio, TX 78229 USA
Titolo Testata:
MECHANISMS OF AGEING AND DEVELOPMENT
fascicolo: 15, volume: 122, anno: 2001,
pagine: 1771 - 1786
SICI:
0047-6374(200110)122:15<1771:EOAAFR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE EXCISION-REPAIR; DIETARY RESTRICTION; CALORIC RESTRICTION; WISTAR RATS; ELECTROCHEMICAL DETECTION; LIPID-PEROXIDATION; PROTEIN OXIDATION; DEPENDENT CHANGES; DIFFERENT TISSUES; FREE-RADICALS;
Keywords:
mouse aorta; antioxidant enzyme; oxidative DNA damage; comet assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Guo, ZM Univ Texas, Hlth Sci Ctr, Dept Physiol, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78229 X 78229 USA
Citazione:
Z.M. Guo et al., "Effects of age and food restriction on oxidative DNA damage and antioxidant enzyme activities in the mouse aorta", MECH AGE D, 122(15), 2001, pp. 1771-1786

Abstract

In this study, DNA damage in mouse aortic cells was measured using the comet assay. The tail moment of the comet assay in aortic cells obtained from 26-month-old mice fed ad libitum (O) was significantly increased as compared to 6-month-old mice fed ad libitum (Y-AL) after the cells were incubated with formamidopyrimidine-DNA glycosylase (Fpg), which specifically recognizes oxidized purines, endonuclease III (Endo III), which specifically recognizes oxidized pyrimidines, or the combination of Endo III and Fpg. The tailmoment in aortic cells obtained from 26-month-old mice fed a food-restricted diet (O-FR) was significantly reduced as compared to O-AL mice after thecells were incubated with the combination of Endo III and Fpg. These results indicate that oxidative DNA lesions, i.e. the Endo III- and Fpg-sensitive sites, increase with age in mouse aortic cells and that FR attenuates theage-related increase in oxidative DNA damage. To determine if the changes in oxidative DNA damage in mouse aortic cells are related to the antioxidant status in these cells, we measured the activities of Cu/Zn-superoxide dismutase (SOD), Mn-SOD, extracellular-SOD, catalase and glutathione peroxidase-1 in the mouse aorta, We observed that the activities of all antioxidant enzymes studied were significantly increased with age and that FR attenuated the age-related increase. These data indicate that the age-related increase and FR-induced decrease in oxidative DNA damage, i.e. the Endo III- and Fpg-sensitive sites, in mouse aortic cells is not due to alteration of the antioxidant defense system. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:16:55