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Titolo:
Glycidol degrades scrapie mouse prion protein
Autore:
Yamamoto, M; Horiuchi, M; Ishiguro, N; Shinagawa, M; Matsuo, T; Kaneko, K;
Indirizzi:
Obihiro Univ Agr & Vet Med, Dept Vet Med, Lab Vet Publ Hlth, Obihiro, Hokkaido 0808555, Japan Obihiro Univ Agr & Vet Med Obihiro Hokkaido Japan 0808555 0808555, Japan Obihiro Univ Agr & Vet Med, Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan Obihiro Univ Agr & Vet Med Obihiro Hokkaido Japan 0808555 0808555, Japan Nihon Pharmaceut Co Ltd, Tokyo Res Lab, Chiba 2860825, Japan Nihon Pharmaceut Co Ltd Chiba Japan 2860825 es Lab, Chiba 2860825, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 9, volume: 63, anno: 2001,
pagine: 983 - 990
SICI:
0916-7250(200109)63:9<983:GDSMPP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-BIOLOGY; DISEASES; CARCINOGENICITY; INACTIVATION; AGENT; SHEEP;
Keywords:
decontamination; gycidol; prion; scrapie;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Shinagawa, M Obihiro Univ Agr & Vet Med, Dept Vet Med, Lab Vet Publ Hlth, Inada Cho, Obihiro, Hokkaido 0808555, Japan Obihiro Univ Agr & Vet Med Inada Cho Obihiro Hokkaido Japan 0808555
Citazione:
M. Yamamoto et al., "Glycidol degrades scrapie mouse prion protein", J VET MED S, 63(9), 2001, pp. 983-990

Abstract

Agents of transmissible spongiform encephalopathy (prion) are known to be extremely resistant to physicochemical inactivation procedures such as heat, radiation, chemical disinfectants such as detergents, alcohols, glutaraldehyde, formalin, and so on. Because of its remarkable resistance, it is difficult to inactivate prion. Chemical inactivation seems to be a practical method because it is applicable to large or fixed surfaces and complicated equipment. Here, three epoxides: beta -propiolactone, propylene oxide, and glycidol (GLD) were examined of their inactivation ability against scrapie-mouse prion protein (PrPSc) under various conditions of chemical concentration, incubation time, and temperature. Among these chemicals, GLD worked most effectively and degraded PrP into small fragments. As a result of the bioassay, treatment with 3% GLD for 5 hr and 5% GLD for 2, 5 hr or 12 hr at room temperature prolonged the mean incubation time by 44, 30, 110 and 73 days, respectively. From dose-incubation time standard curve, the decrease in infectivity titers were estimated as 10(3) or more. Therefore, degradation of PrPSc by GLD decreased the scrapie infectivity. It is also suggested that pH and salt concentrations influence the effect of GLD. Although further study is necessary to determine the optimal condition, GLD may be a potential prion disinfectant.

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Documento generato il 19/09/20 alle ore 18:06:06