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Titolo:
Cloning and characterization of excitatory amino acid transporters GLT-1 and EAAC1 in canine brain
Autore:
Sato, K; Inaba, M; Baba, K; Tamahara, S; Koshino, I; Hikasa, Y; Ono, K; Kagota, K;
Indirizzi:
Tottori Univ, Fac Agr, Dept Vet Internal Med, Tottori 6808553, Japan Tottori Univ Tottori Japan 6808553 Internal Med, Tottori 6808553, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Lab Vet Clin Pathobiol, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Vet Clin Pathobiol, Tokyo 1138657, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 9, volume: 63, anno: 2001,
pagine: 997 - 1002
SICI:
0916-7250(200109)63:9<997:CACOEA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; GLUTAMATE TRANSPORTER; RAT-BRAIN; EXPRESSION; MICE; IMMUNOCYTOCHEMISTRY; NEURODEGENERATION; CYSTEINE; MUTATION; MUTANTS;
Keywords:
brain; canine; cDNA cloning; excitatory amino acid transporter; glutamate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, K Tottori Univ, Fac Agr, Dept Vet Internal Med, Tottori 6808553, Japan Tottori Univ Tottori Japan 6808553 l Med, Tottori 6808553, Japan
Citazione:
K. Sato et al., "Cloning and characterization of excitatory amino acid transporters GLT-1 and EAAC1 in canine brain", J VET MED S, 63(9), 2001, pp. 997-1002

Abstract

Excitatory amino acid transporters play important roles in termination of glutamatergic neurotransmission and protection of neurons from the excitotoxicity of glutamate in the central nervous system. We herein report isolation of cDNA clones of two distinct excitatory amino acid transporters, GLT-1and EAAC1, from canine brain cortex by PCR-based cloning, and characterization of these transporter subtypes. Canine GLT-1 and EAAC1 exhibited Na+-dependent glutamate transport with high affinities in a Xenopus oocyte expression system. Despite the similarity in transport kinetics and in the predicted primary structures, GLT-1, EAAC1, and the previously identified GLAST showed different sensitivities to several structural analogues of L-glutamate. In addition, transcripts of these transporter subtypes showed distinct regional distribution in the brain in RT-PCR analysis, suggesting that excitatory amino acid transporters have distinct physiological and pathophysiological roles in the brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:27:15