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Titolo:
Endorphin and enkephalin ameliorate excessive synovial cell functions in patients with rheumatoid arthritis
Autore:
Takeba, Y; Suzuki, N; Kaneko, A; Asai, T; Sakane, T;
Indirizzi:
St Marianna Univ, Sch Med, Dept Immunol, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168511 Kanagawa 2168511, Japan St Marianna Univ, Sch Med, Dept Med, Kawasaki, Kanagawa 2168511, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168511 Kanagawa 2168511, Japan Nagoya Natl Hosp, Dept Orthoped Surg, Nagoya, Aichi, Japan Nagoya Natl Hosp Nagoya Aichi Japan Orthoped Surg, Nagoya, Aichi, Japan
Titolo Testata:
JOURNAL OF RHEUMATOLOGY
fascicolo: 10, volume: 28, anno: 2001,
pagine: 2176 - 2183
SICI:
0315-162X(200110)28:10<2176:EAEAES>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELTA-OPIOID RECEPTOR; BETA-ENDORPHIN; INFLAMED TISSUE; IMMUNE-SYSTEM; CHROMOSOMAL ASSIGNMENT; HUMAN-MONOCYTES; INFLAMMATION; PEPTIDES; EXPRESSION; ANTINOCICEPTION;
Keywords:
rheumatoid arthritis; synovial cells; methionine-enkephalin; leucine-enkephalin; beta-endorphin; creb;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, N St Marianna Univ, Sch Med, Dept Immunol, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168511 2168511, Japan
Citazione:
Y. Takeba et al., "Endorphin and enkephalin ameliorate excessive synovial cell functions in patients with rheumatoid arthritis", J RHEUMATOL, 28(10), 2001, pp. 2176-2183

Abstract

Objective. To determine whether endorphin (END) and enkephalin (ENK) modulate excessive synovial cell functions in patients with rheumatoid arthritis(RA). Methods. Effects of leucine-enkephalin (leu-ENK), methionine-enkephalin (met-ENK), and beta -endorphin (END) on proinflammatory cytokine and matrix metalloproteinase (MMP) production by RA synovial cells were analyzed by immunoblotting, and their mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) using limiting dilution of complementary DNA. Expression of opioid receptors on RA synovial cells was assessed by immunohistochemical staining, radioreceptor assay, and RT-PCR. Results. Leu-ENK, met-ENK, and END inhibited tumor necrosis factor-alpha and interleukin 1 beta production at the level of mRNA expression. ENK and END inhibited MMP-9 production and its enzymatic activity by RA synovial cells. The p-subtype opioid receptor was expressed in the RA synovial lining and sublining cells. Radioreceptor assay suggested expression of high affinity receptor for END on RA synovial cells. The p-subtype opioid receptor-specific antagonist, naloxone, reversed the inhibitory effect of the opioid peptides. The opioid peptides inhibited nuclear translocation and phosphorylation of the transcription factor, cyclic AMP responsive element binding protein (CREB) in RA synovial cells. Conclusion. Leu-ENK, met-ENK, and END inhibited excessive RA synovial cellfunctions in vitro. The opioid hormones may have not only antinociceptive action, but also antiinflammatory effects on synovitis itself in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:56:18