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Titolo:
Expression pattern and gene structure of phenylalanine ammonia-lyase in Pharbitis nil
Autore:
Nakazawa, A; Nozue, M; Yasuda, H; Takeba, G; Kubo, H;
Indirizzi:
Shinshu Univ, Fac Sci, Dept Biol, Matsumoto, Nagano 3908621, Japan ShinshuUniv Matsumoto Nagano Japan 3908621 sumoto, Nagano 3908621, Japan Shinshu Univ, Fac Text Sci & Technol, Dept Appl Biol, Ueda, Nagano 3868567, Japan Shinshu Univ Ueda Nagano Japan 3868567 Biol, Ueda, Nagano 3868567, Japan Kyoto Prefectural Univ, Dept Life Sci, Lab Appl Biol, Kyoto 6060832, JapanKyoto Prefectural Univ Kyoto Japan 6060832 pl Biol, Kyoto 6060832, Japan
Titolo Testata:
JOURNAL OF PLANT RESEARCH
fascicolo: 1115, volume: 114, anno: 2001,
pagine: 323 - 328
SICI:
0918-9440(200109)114:1115<323:EPAGSO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT DEFENSE GENES; FUNGAL ELICITOR; ARABIDOPSIS-THALIANA; MESSENGER-RNAS; UV-LIGHT; PROTEIN; FLOWERS; ANTHOCYANIN; PROMOTER; BINDING;
Keywords:
anthocyanin; Pharbitis nil; phenylalanine ammonia-lyase; phytochrome; red light;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kubo, H Shinshu Univ, Fac Sci, Dept Biol, Matsumoto, Nagano 3908621, JapanShinshu Univ Matsumoto Nagano Japan 3908621 Nagano 3908621, Japan
Citazione:
A. Nakazawa et al., "Expression pattern and gene structure of phenylalanine ammonia-lyase in Pharbitis nil", J PLANT RES, 114(1115), 2001, pp. 323-328

Abstract

Anthocyanin accumulation, phenylalanine ammonia-lyase (PAL) activity and PAL gene expression were examined in flower buds and irradiated hypocotyls in Pharbitis nil. PAL activity and transcript levels were correlated with the accumulation of anthocyanin. Both in flower buds and hypocotyls, transcript levels, PAL activity, and then the amount of anthocyanin, increased. ThePAL transcript was abundant in flower buds for a few days before flower opening. But the increase in PAL transcript induced by irradiation was temporal in hypocotyls. Phytochrome was shown to be involved in inducing the accumulation of anthocyanin in hypocotyls. To examine the mechanism regulating the expression of the PAL gene, the gene was cloned and sequenced, and the promoter region was compared with that of other PALs. The gene had two exons separated by an intron of 989 bp with consensus sequences at the intron/exon border. The predicted primary structure of the PAL protein consists of 711 amino acids. The promoter region was AT-rich and there were sequences similar to box 1, box 2, an AT-1 binding site and a G box. The role of PAL in the accumulation of anthocyanin is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 20:40:02