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Titolo:
Functional characterization of the human prion protein promoter in neuronal and endothelial cells
Autore:
Funke-Kaiser, H; Theis, S; Behrouzi, T; Thomas, A; Scheuch, K; Zollmann, FS; Paterka, M; Paul, M; Orzechowski, HD;
Indirizzi:
Free Univ Berlin, Klinikum Benjamin Franklin, Dept Clin Pharmacol, Inst Clin Pharmacol & Toxicol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 Toxicol, D-12200 Berlin, Germany
Titolo Testata:
JOURNAL OF MOLECULAR MEDICINE-JMM
fascicolo: 9, volume: 79, anno: 2001,
pagine: 529 - 535
SICI:
0946-2716(200109)79:9<529:FCOTHP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CREUTZFELDT-JAKOB-DISEASE; TRANSCRIPTION FACTORS; MESSENGER-RNA; GENE-EXPRESSION; TRANSGENIC MICE; NERVOUS-SYSTEM; PRP GENE; SCRAPIE; VARIANT; PRECURSOR;
Keywords:
human prion protein promoter; reporter gene assay; transcriptional start sites; neuronal and endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Orzechowski, HD Free Univ Berlin, Klinikum Benjamin Franklin, Dept Clin Pharmacol, Inst Clin Pharmacol & Toxicol, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200
Citazione:
H. Funke-Kaiser et al., "Functional characterization of the human prion protein promoter in neuronal and endothelial cells", J MOL MED-J, 79(9), 2001, pp. 529-535

Abstract

Human prion diseases such as Creutzfeld-Jakob disease and kuru are of major medical and biological importance because of their fatal course, epidemicpotential, and unique pathophysiology. Endogenous expression of the normalcellular prion protein (PrPC) is necessary for infection and prion replication. However, knowledge of human PrPC gene regulation is rudimentary. We therefore cloned1543 bp of the 5' untranslated and promoter region of the PrP gene. Using transient transfection assays, the full-length promoter and serial deletion mutants subcloned in a luciferase reporter vector were analyzed in neuronal (KELLY) and endothelial (EA.hy926) cell lines, which both express PrPC as shown by RT/PCR. Analysis of promoter constructs in KELLY cells indicated two activating regions at -131/-284 and -1303/-1543, relativeto the 3'-terminal end of exon 1, and also two repressing elements at -254/-567 and -567/-909 in neuronal cells. In EA.hy926 cells, activating elements were identified at -131/-284 and -284/-567, and one repressing region was localized at -567/-909. In addition, transcriptional start sites were determined by 5'-RACE reaction and RNase protection assay, revealing one majortranscriptional start site located at -47 (in KELLY cells), -53 (in human thalamus) and at about -55 (in EA.hy926 cells).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 09:03:24