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Titolo:
Capillary electrophoretic behaviour and determination of enoxacin in pharmaceutical preparations and human serum
Autore:
Tuncel, M; Dogrukol-Ak, D; Senturk, Z; Ozkan, SA; Aboul-Enein, H;
Indirizzi:
Univ Anadolu, Fac Pharm, Dept Analyt Chem, TR-26470 Eskisehir, Turkey UnivAnadolu Eskisehir Turkey TR-26470 Chem, TR-26470 Eskisehir, Turkey Gazi Univ, Fac Pharm, Dept Analyt Chem, TR-06330 Andara, Turkey Gazi UnivAndara Turkey TR-06330 pt Analyt Chem, TR-06330 Andara, Turkey Ankara Univ, Fac Pharm, Dept Analyt Chem, TR-06100 Ankara, Turkey Ankara Univ Ankara Turkey TR-06100 Analyt Chem, TR-06100 Ankara, Turkey King Faisal Specialist Hosp & Res Ctr, Dept Biol & Med Res, Pharmaceut Anal Lab, Riyadh 11211, Saudi Arabia King Faisal Specialist Hosp & Res Ctr Riyadh Saudi Arabia 11211 i Arabia
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 16, volume: 24, anno: 2001,
pagine: 2455 - 2467
SICI:
1082-6076(2001)24:16<2455:CEBADO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN-PLASMA; URINE; FLUOROQUINOLONES; METABOLITE; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Aboul-Enein, H Univ Anadolu, Fac Pharm, Dept Analyt Chem, TR-26470 Eskisehir, Turkey Univ Anadolu Eskisehir Turkey TR-26470 Eskisehir, Turkey
Citazione:
M. Tuncel et al., "Capillary electrophoretic behaviour and determination of enoxacin in pharmaceutical preparations and human serum", J LIQ CHR R, 24(16), 2001, pp. 2455-2467

Abstract

A validated capillary electrophoretic method with UV detection is described for the determination of enoxacin [ENX] in pharmaceutical preparation andhuman serum. The experiments were carried out in a fused-silica capillary (ID=75 tm, total 88 cm, effective 58 cm length) using a 20 mM borate bufferat pH 8.6, applying a potential of 30 kV, l s of injection. Acetylpipedimic acid was used as an internal standard (IS) and the detection was performed at 265 nm. The t(M)+/- RSD% of ENX and IS were 4.8 +/-0.9 and 5.5 +/-1.2 minutes, respectively. A well-correlated calibration equation was obtained in the range of 3.1x10(-6)-3.1x10(5) M ENX. Limit of detection (LOD) was 3.5x10(-6) M (S/N = 3). A modified reversed-phase HPLC was also conducted using a C-18 ODS column for the analysis of ENX to compare to its applicability with the CE method. An isocratic elution was performed using a mobile phase of 10 mM phosphatebuffer (pH 4.0) and acetonitrile (85:15;v/v) detecting at 260 nm. The determination of ENX in the pharmaceutical tablet formulation was carried out by both methods and the results of a single tablet (as mg with their RSD% values) was found to be 421.4 +/-1.0 and 415.9 +/-0.9 by CE and HPLC, respectively. ENX analysis was also performed by a standard addition method in serum, and the recoveries were found to be 89.7 +/-0.6 (CE) and 78.8 /-4.9 (HPLC). It was concluded that capillary electrophoresis for the determination of ENX is a promising method for routine analysis and pharmacokinetic and bioavailability studies.

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Documento generato il 19/01/20 alle ore 00:23:34