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Titolo:
Non-invasive observation of repeated adenoviral GFP gene delivery to the anterior segment of the monkey eye in vivo
Autore:
Borras, T; Gabelt, BT; Klintworth, GK; Peterson, JC; Kaufman, PL;
Indirizzi:
Duke Univ, Med Ctr, Dept Ophthalmol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Ctr, Dept Ophthalmol, Durham, NC 27710 USA Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI 53792 USA Univ Wisconsin Madison WI USA 53792 l & Visual Sci, Madison, WI 53792 USA
Titolo Testata:
JOURNAL OF GENE MEDICINE
fascicolo: 5, volume: 3, anno: 2001,
pagine: 437 - 449
SICI:
1099-498X(200109/10)3:5<437:NOORAG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN TRABECULAR MESHWORK; REPEATED LUNG EXPOSURE; COLLAGEN TYPE-I; MEDIATED TRANSFER; NONHUMAN-PRIMATES; CILIARY MUSCLE; CYNOMOLGUS MONKEY; AQUEOUS-HUMOR; TRANSGENE EXPRESSION; UVEOSCLERAL OUTFLOW;
Keywords:
trabecular meshwork; adenoviral vectors; repeated gene transfer; monkeys; glaucoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Borras, T Duke Univ, Med Ctr, Dept Ophthalmol, Wadsworth Bldg,Erwin Rd,Box3802, Durham, NC 27710 USA Duke Univ Wadsworth Bldg,Erwin Rd,Box 3802 Durham NC USA 27710 A
Citazione:
T. Borras et al., "Non-invasive observation of repeated adenoviral GFP gene delivery to the anterior segment of the monkey eye in vivo", J GENE MED, 3(5), 2001, pp. 437-449

Abstract

Background Glaucoma is a group of chronic eye diseases often associated with an elevated intraocular pressure (IOP). If not controlled, the conditionleads to blindness. The eye tissue responsible for maintaining aqueous humor. resistance and thus normal IOP is the trabecular meshwork (TM). Adenoviral vectors are capable of transducing the TM in several rodent species. Because, of the relevance of the non-human primate model in the study of glaucoma, gene transfer to the eyes of cynomolgus monkeys was investigated. Methods Four cynomolgus monkeys were injected with AdenoGFP into the anterior chamber: two monkeys received 10(9) pfu and the other two 10(7) pfu. One monkey received four consecutive injections into the same eye (10(7) pfu in each injection) over a 7-month period. In vivo gene transfer (fluorescence) and IOP were evaluated by standard clinical ophthalmic instruments (slit lamp biomicroscopy, gonioscopy and tonometry). Histopathology and cellular distribution were assessed postmortem. Results The first injection of the lower viral dose resulted in marked TM-preferred gene transfer visible non-invasively by in vivo gonioscopy. The expression of the trans-ene lasted for 3-4 weeks with little or no signs of clinical inflammation. Gene transfer was achieved on three sequential occasions (3-4 weeks each) but failed and induced substantial, albeit reversible, corneal abnormalities on the fourth occasion. Conclusions Gene transfer to the TM and cornea can be monitored noninvasively in non-human primates, allowing correlation of gene transfer with physiological parameters. Because of ocular immune privilege, repeated anterior chamber administrations of adenoviral vectors expressing appropriate genes may have therapeutic potential for glaucoma. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:08:41