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Titolo:
The sterol composition of Trypanosoma cruzi changes after growth in different culture media and results in different sensitivity to digitonin-permeabilization
Autore:
Rodrigues, CO; Catisti, R; Uyemura, SA; Vercesi, AE; Lira, R; Rodriguez, C; Urbina, JA; Docampo, R;
Indirizzi:
Univ Illinois, Dept Pathobiol, Mol Parasitol Lab, Urbana, IL 61802 USA Univ Illinois Urbana IL USA 61802 Mol Parasitol Lab, Urbana, IL 61802 USA Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, Sao Paulo, Brazil Univ Estadual Campinas Sao Paulo Brazil t Patol Clin, Sao Paulo, Brazil Inst Venezolano Invest Cient, Ctr Biofis & Bioquim, Lab Quim Biol, Caracas, Venezuela Inst Venezolano Invest Cient Caracas Venezuela Biol, Caracas, Venezuela
Titolo Testata:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
fascicolo: 5, volume: 48, anno: 2001,
pagine: 588 - 594
SICI:
1066-5234(200109/10)48:5<588:TSCOTC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-STREAM FORMS; ISOLATED-HEPATOCYTES; MITOCHONDRIA INSITU; CALCIUM HOMEOSTASIS; CA2+ TRANSPORT; BRUCEI; CELLS; RELEASE; PYROPHOSPHATASE; ACIDOCALCISOMES;
Keywords:
cholesterol; membrane potential; mitochondria; oxidative phosphorylation; respiration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Docampo, R Univ Illinois, Dept Pathobiol, Mol Parasitol Lab, Urbana, IL 61802 USA Univ Illinois Urbana IL USA 61802 ol Lab, Urbana, IL 61802 USA
Citazione:
C.O. Rodrigues et al., "The sterol composition of Trypanosoma cruzi changes after growth in different culture media and results in different sensitivity to digitonin-permeabilization", J EUKAR MIC, 48(5), 2001, pp. 588-594

Abstract

Respiration, oxidative phosphorylation, and the corresponding changes in membrane potential (Delta Psi) of Trypanosoma cruzi epimastigotes grown either in liver infusion-tryptose (LIT) or brain heart infusion (BHI) culture medium were assayed in situ using digitonin to render their plasma membrane permeable to succinate, ADP, safranine O, and other small molecules. When the cells were permeabilized with 64 muM digitionin, a concentration previously used with epimastigotes, the ability of the cells grown in LIT medium to sustain oxidative phosphorylation was demonstrated by the detection of anoligomycin-sensitive decrease in mitochondrial membrane potential induced by ADP. In contrast, the cells grown in BHI medium were not able to sustaina stable membrane potential and did not respond to ADP addition. Analyses of oxygen consumption by these permeabilized cells indicated that the rate of basal respiration, which was similar in both cell types, was significantly decreased by 64 muM digitonin. Addition of ADP to the permeabilized cells grown in LIT medium promoted an oligomycin-sensitive transition from resting to phosphorylating respiration in contrast to the cells grown in BHI medium, whose respiration decreased steadily and did not respond either to ADP or CCCP. Titration of the cells grown in BIR medium with different digitonin concentrations indicated that their mitochondria have higher sensitivity to digitonin than those grown in LIT medium. Analysis of the sterol composition of epimastigotes grown in the two different media showed a higher percentage of cholesterol in total and mitochondrial extracts of epimastigotes grown in BIH medium as compared to those grown in LIT medium, suggesting the involvement of this sterol in their increased sensitivity to digitonin-permeabilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:52:53