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Titolo:
Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts
Autore:
Abe, T; Abe, Y; Aida, Y; Hara, Y; Maeda, K;
Indirizzi:
Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Sect Periodontol,Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 tol,Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Sect Clin Oral Med Biol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Oral Med Biol, Fukuoka 8128582, Japan Nagasaki Univ, Sch Dent, Dept Periodontol, Nagasaki 852, Japan Nagasaki Univ Nagasaki Japan 852 , Dept Periodontol, Nagasaki 852, Japan
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 2, volume: 189, anno: 2001,
pagine: 144 - 151
SICI:
0021-9541(200111)189:2<144:EMRIOA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY PERIODONTAL-DISEASE; HUMAN SKIN FIBROBLASTS; GROWTH-FACTOR; GINGIVAL FIBROBLASTS; OSTEOBLASTIC DIFFERENTIATION; MC3T3-E1 CELLS; COLLAGEN; FIBRONECTIN; SECRETION; INTEGRINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, T Kyushu Univ, Fac Dent Sci, Div Oral Rehabil, Sect Periodontol,Higashi Ku, Maidashi 3-1-1, Fukuoka 8128582, Japan Kyushu Univ Maidashi 3-1-1 Fukuoka Japan 8128582 ka 8128582, Japan
Citazione:
T. Abe et al., "Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts", J CELL PHYS, 189(2), 2001, pp. 144-151

Abstract

During wound healing and inflammation, fibroblasts express elevated alkaline phosphatase (ALP), but are not in contact with collagen fibrils in the fibronectin (FN)-rich granulation tissue. We hypothesized that the extracellular matrix (ECM) environment might influence the induction of ALP in fibroblasts, Here we tested this hypothesis by studying the ALP-inductive response of normal human gingival, p fibroblasts to ascorbic acid (AsA). AsA induced ALP activity and protein in cells in conventional monolayer culture. This induction was inhibited by blocking-antibodies to the FN receptor alpha5beta1 integrin and by the proline analog 3,4-dehydroproline (DHP). DHP prevented cells from arranging FN fibrils into a pericellular network and reduced the activity of cell spreading on FN. Plating of cells on FN facilitated the up-regulation by AsA of ALP expression, but did not substitute for AsA. In contrast, AsA did not cause ALP induction in cells cultured on and inpolymerized type I collagen gets. Collagen fibrils inhibited the upregulation by AsA of ALP expression in cells plated on FN. These results indicate that the ECM regulates the induction of ALP expression by AsA in fibroblasts: FN enables them to express ALP in response to AsA through interaction with integrin alpha5 beta1, whereas type I collagen fibrils cause the suppression of ALP expression and overcome FN. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:09:44