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Titolo:
Dense core secretory vesicles revealed as a dynamic Ca2+ store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera
Autore:
Mitchell, KJ; Pinton, P; Varadi, A; Tacchetti, C; Ainscow, EK; Pozzan, T; Rizzuto, R; Rutter, GA;
Indirizzi:
Univ Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England Univ Ferrara, Expt & Diagnost Med Sect Gen Pathol, I-44100 Ferrara, Italy Univ Ferrara Ferrara Italy I-44100 ct Gen Pathol, I-44100 Ferrara, Italy Univ Padua, CNR, Ctr Study Biol Membranes, I-35121 Padua, Italy Univ Padua Padua Italy I-35121 tudy Biol Membranes, I-35121 Padua, Italy Univ Genoa, Sch Med, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132Univ Genoa, Sch Med, I-16132 Genoa, Italy
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 1, volume: 155, anno: 2001,
pagine: 41 - 51
SICI:
0021-9525(20011001)155:1<41:DCSVRA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC BETA-CELLS; CYCLIC ADP-RIBOSE; GREEN FLUORESCENT PROTEINS; ENDOPLASMIC-RETICULUM; INSULIN-SECRETION; PLASMA-MEMBRANE; INOSITOL TRISPHOSPHATE; RYANODINE RECEPTOR; ADENINE-NUCLEOTIDES; PROPERTIES DISTINCT;
Keywords:
calcium; secretory vesicle; insulin; ryanodine receptor; aequorin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
89
Recensione:
Indirizzi per estratti:
Indirizzo: Rutter, GA Univ Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England UnivBristol Bristol Avon England BS8 1TD 8 1TD, Avon, England
Citazione:
K.J. Mitchell et al., "Dense core secretory vesicles revealed as a dynamic Ca2+ store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera", J CELL BIOL, 155(1), 2001, pp. 41-51

Abstract

The role of dense core secretory vesicles in the control of cytosolic-freeCa2+ concentrations ([Ca2+](c)) in neuronal and neuroendocrine cells is enigmatic. By constructing a vesicle-associated membrane protein 2-synaptobrevin.aequorin chimera, we show that in clonal pancreatic islet beta -cells: (a) increases in [Ca2+](c) cause a prompt increase in intravesicular-free Ca2+ concentration ([Ca2+](SV)), which is mediated by a P-type Ca2+-ATPase distinct from the sarco(endo) plasmic reticulum Ca2+-ATPase, but which may be related to the PMR1/ATP2C1 family of Ca2+ pumps; (b) steady state Ca2+ concentrations are 3-5-fold lower in secretory vesicles than in the endoplasmic reticulum (ER) or Golgi apparatus, suggesting the existence of tightly bound and more rapidly exchanging pools of Ca2+; (C) inositol (1,4,5) trisphosphate has no impact on [Ca2+](SV) in intact or permeabilized cells; and (d) ryanodine receptor (RyR) activation with caffeine or 4-chloro-3-ethylphenol in intact cells, or cyclic ADPribose in permeabilized cells, causes a dramatic fall in [Ca2+](SV). Thus, secretory vesicles represent a dynamic Ca2+ store in neuroendocrine cells, whose characteristics are in part distinct from the ER/Golgi apparatus. The presence of RyRs on secretory vesicles suggests that local Ca2+-induced Ca2+ release from vesicles docked at the plasma membrane could participate in triggering exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 18:37:34