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Titolo:
C1q-independent activation of neutrophils by immunoglobulin M-coated surfaces
Autore:
Wettero, J; Bengtsson, T; Tengvall, P;
Indirizzi:
Linkoping Univ, Dept Phys & Measurement Technol, Appl Phys Lab, SE-58183 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58183 Lab, SE-58183 Linkoping, Sweden Linkoping Univ, Fac Hlth Sci, Dept Hlth & Environm, Div Med Microbiol, SE-58185 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58185 iol, SE-58185 Linkoping, Sweden
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 4, volume: 57, anno: 2001,
pagine: 550 - 558
SICI:
0021-9304(200112)57:4<550:CAONBI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-GAMMA-RIII; IN-VITRO; RESPIRATORY BURST; INFLAMMATORY RESPONSES; COMPLEMENT ACTIVATION; HOCL GENERATION; SHEAR-STRESS; HUMAN-SERUM; IGG; C1Q;
Keywords:
complement activation; immunoglobulins; actin cytoskeleton; neutrophil respiratory burst; protein adsorption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Wettero, J Linkoping Univ, Dept Phys & Measurement Technol, Appl Phys Lab,SE-58183 Linkoping, Sweden Linkoping Univ Linkoping Sweden SE-58183 83 Linkoping, Sweden
Citazione:
J. Wettero et al., "C1q-independent activation of neutrophils by immunoglobulin M-coated surfaces", J BIOMED MR, 57(4), 2001, pp. 550-558

Abstract

Neutrophil granulocytes are known to rapidly adhere and undergo frustratedphagocytosis upon contact with immunoglobulin and/or complement protein opsonized artificial surfaces. In this study, we examined the relation between serum protein deposition and human neutrophil activation on hydrophobic glass and silicon model surfaces that were coated with immunoglobulin G or M(IgG/IgM), both initiators of the classical complement pathway. Protein adsorption from normal human serum (NHS) was quantified with null-ellipsometry combined with antibody techniques. The neutrophil oxygen radical production was registered by luminol-amplified chemiluminescence (CL) and the morphology, as well as changes in the content of filamentous actin (F-actin), were documented by fluorescence microscopy. Complement factor 3 (C3) bound toboth IgG- and IgM-coated surfaces, but surprisingly C1q was found only on IgG-coated surfaces. Both immunoglobulins triggered complement dependent neutrophil activation. However, CL and F-actin accumulation were found sensitive to the presence of Clq in the serum only at the IgG-coated surface. We suggest that spontaneously adsorbed IgM activates the complement system andinteracts with neutrophils by C1q-independent mechanisms. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:16:28