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Titolo:
The acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular utilization
Autore:
Faergeman, NJ; Black, PN; Zhao, XD; Knudsen, J; DiRusso, CC;
Indirizzi:
Albany Med Coll, Ctr Cardiovasc Sci, Albany, NY 12208 USA Albany Med CollAlbany NY USA 12208 Cardiovasc Sci, Albany, NY 12208 USA Univ So Denmark, Inst Biochem & Mol Biol, DK-5220 Odense M, Denmark Univ So Denmark Odense Denmark M m & Mol Biol, DK-5220 Odense M, Denmark
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 40, volume: 276, anno: 2001,
pagine: 37051 - 37059
SICI:
0021-9258(20011005)276:40<37051:TASEWF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN N-MYRISTOYLATION; PEROXISOMAL PROTEINS; BINDING-PROTEIN; TRANSCRIPTION FACTORS; STATIONARY-PHASE; OLE1 GENE; YEAST; MEMBRANE; METABOLISM; DISRUPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: DiRusso, CC Albany Med Coll, Ctr Cardiovasc Sci, MC-8,47 New Scotland Ave,Albany, NY 12208 USA Albany Med Coll MC-8,47 New Scotland Ave Albany NY USA 12208 A
Citazione:
N.J. Faergeman et al., "The acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular utilization", J BIOL CHEM, 276(40), 2001, pp. 37051-37059

Abstract

Exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic utilization. In the yeast Saccharomyces cerevisiae, the activation of these compounds prior to metabolic utilization proceeds through the fatty acyl-CoA synthetases Faa1p and Faa4p. Faa1p or Faa4p are essential for long-chain fatty acid import, suggesting that one or both of these enzymes are components of the fatty acid transport system, which also includes Fat1p. By monitoring the intracellular accumulation of the fluorescent long-chain fatty acid analogue 4,4-difluoro-5-methyl-4-bora-3a,4a-diazas-indacene-3-dodecanoic acid, long-chain fatty acid transport was shown to be severely restricted in a faa1 Delta faa4 Delta strain. These data established for the first time a mechanistic linkage between the import and activation of exogenous fatty acids in yeast. To investigate this linkage further, oleoyl CoA levels were defined following incubation of wild type and mutant cells with limiting concentrations of exogenous oleate. These studies demonstrated oleoyl CoA levels were reduced to less than 10% wild-type levels infaa1 Delta and faa1 Delta faa4 Delta strains. Defects in metabolic utilization and intracellular trafficking were also found in the fatty acyl-CoA synthetase-deficient strains. The faa1 Delta faa4 Delta strain had a marked reduction in endogenous acyl-CoA pools, suggesting these enzymes play a rolein maintenance of endogenous acyl-CoA pools, metabolism and trafficking. In addition, this strain had levels of in vivo beta -oxidation of exogenous oleate reduced 3-fold when compared with the isogenic parent. Northern analyses demonstrated an additional defect in fatty acid trafficking as FAA1 orFAA4 were required for the transcriptional regulation of the genes encoding the peroxisomal enzymes acyl-CoA oxidase (POX1) and medium-chain acyl-CoAsynthetase (FAA2). These data support the hypothesis that fatty acyl-CoA synthetase (Faa1p or Faa4p) functions as a component of the fatty acid import system by linking import and activation of exogenous fatty acids to intracellular utilization and signaling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:46:53