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Titolo:
Effect of incubation temperature and salinity on expression of the outer membrane protein profile of Edwardsiella tarda
Autore:
Darwish, AM; Newton, JC; Plumb, JA;
Indirizzi:
Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Med, Dept Pathobiol, Auburn, AL 36849 USA Auburn Univ, Coll Agr, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Allied Aquacultures, Auburn, AL 36849 USA
Titolo Testata:
JOURNAL OF AQUATIC ANIMAL HEALTH
fascicolo: 3, volume: 13, anno: 2001,
pagine: 269 - 275
SICI:
0899-7659(200109)13:3<269:EOITAS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRON-REGULATED HEMOLYSIN; INFECTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Darwish, AM Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA Harry K Dupree Stuttgart Natl Aquaculture Res Ctr Stuttgart AR USA 72160
Citazione:
A.M. Darwish et al., "Effect of incubation temperature and salinity on expression of the outer membrane protein profile of Edwardsiella tarda", J AQUAT A H, 13(3), 2001, pp. 269-275

Abstract

Outer membrane proteins (OMP) of 10 isolates of Edwardsiella tarda were compared by sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis. The OMP profile of the type strain E. tarda ATCC 15947 cultured at 25 degreesC had five major protein bands of 40, 36.5, 34, 28.5, and 25 kDa and alarge number of minor proteins ranging in size from approximately 10 to 120 kDa. Differences between the OMP profiles of the isolates of E. tarda included the inconsistent presence of the 34- or 36.5-kDa proteins in five isolates of E. tarda and two major bands of 47 and 44 kDa that were present inonly two isolates of E. tarda. There were no differences in the outer membrane protein profiles of 9 out of 10 isolates of E. tarda incubated at a temperature of 25 degreesC compared with those at 35 degreesC. To evaluate the effect of salinity, 10 isolates of E. tarda were cultured in brain heart infusion broth containing 0.5, 1.5, and 3.0% sodium chloride. Reactions of isolates of E. tarda to the different salinity levels were placed into three groups. The first group expressed more or fewer protein bands at 1.5% sodium chloride. The second group lost major bands at 3% salinity, whereas thethird group had no change in the OMP profile with salinity. The OMP profile differences and the different reactions to salinity levels suggest that the isolates are heterogeneous.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:46:10