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Titolo:
Activation of caspases and mitochondria in FTY720-mediated apoptosis in human T cell line Jurkat
Autore:
Fujino, M; Li, XK; Guo, L; Amano, T; Suzuki, S;
Indirizzi:
Natl Childrens Med Res Ctr, Dept Expt Surg & Bioengn, Setagaya Ku, Tokyo 1548509, Japan Natl Childrens Med Res Ctr Tokyo Japan 1548509 Ku, Tokyo 1548509, Japan Tokyo Univ Agr & Technol, Dept Zootech Sci, Tokyo, Japan Tokyo Univ Agr & Technol Tokyo Japan ol, Dept Zootech Sci, Tokyo, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 11, volume: 1, anno: 2001,
pagine: 2011 - 2021
SICI:
1567-5769(200110)1:11<2011:AOCAMI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSSESSING UNIQUE MECHANISMS; TERM GRAFT ACCEPTANCE; CYTOCHROME-C; LYMPHOCYTE APOPTOSIS; ALLOGRAFT SURVIVAL; PERMEABILITY TRANSITION; NUCLEAR APOPTOSIS; CYCLOSPORINE-A; FTY720; IMMUNOSUPPRESSANT;
Keywords:
FTY720; apoptosis; caspase; mitochondria; Jurkat cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, S Natl Childrens Med Res Ctr, Dept Expt Surg & Bioengn, Setagaya Ku, 3-35-31Taishido, Tokyo 1548509, Japan Natl Childrens Med Res Ctr 3-35-31Taishido Tokyo Japan 1548509
Citazione:
M. Fujino et al., "Activation of caspases and mitochondria in FTY720-mediated apoptosis in human T cell line Jurkat", INT IMMUNO, 1(11), 2001, pp. 2011-2021

Abstract

FTY720, a novel immunosuppressive drug originally derived from a metabolite from Isaria sinclairii, is known to induce apoptosis in lymphocytes. In this study, we investigated the involvement of caspases and mitochondria in FTY720-mediated apoptosis using Jurkat cells, a human T cell line. Our results indicated that FTY720-induced activation of caspases 2, 3, 6, 8, 9 and 10, whereas caspases 1 and 5 were not activated. We also observed in the FTY720-treated cells a loss of mitochondrial membrane potential, a release ofcytochrome c into cytosol and an exposed phosphatidylserine (PS) at the outer surface of the cell membrane. Pretreatment with a peptide inhibitor, benzyloxycarbonyl-Asp-CH2COC-2, 6-dichlorobenzene (Z-Asp-CH2-DCB), prevented apoptosis and externalization of phosphatidylserine, whereas the inhibitor did not prevent the mitochondrial events. This suggests that caspases may play a role downstream of the mitochondrial pathway. Therefore, caspase cascade in FTY720-treated cells may be initiated by activation of mitochondria. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/02/20 alle ore 18:09:24