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Titolo:
Administration of mu-, kappa- or delta(2)-receptor agonists via osmotic minipumps suppresses murine splenic antibody responses
Autore:
Rahim, RT; Meissler, JJ; Cowan, A; Rogers, TJ; Geller, EB; Gaughan, J; Adler, MW; Eisenstein, TK;
Indirizzi:
Temple Univ, Sch Med, Dept Microbiol & Immunol, Philadelphia, PA 19140 USATemple Univ Philadelphia PA USA 19140 Immunol, Philadelphia, PA 19140 USA Temple Univ, Sch Med, Dept Pharmacol, Philadelphia, PA 19140 USA Temple Univ Philadelphia PA USA 19140 armacol, Philadelphia, PA 19140 USA Temple Univ, Sch Med, Ctr Subst Abuse Res, Philadelphia, PA 19140 USA Temple Univ Philadelphia PA USA 19140 use Res, Philadelphia, PA 19140 USA Temple Univ, Sch Med, Dept Physiol, Philadelphia, PA 19140 USA Temple Univ Philadelphia PA USA 19140 Physiol, Philadelphia, PA 19140 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 11, volume: 1, anno: 2001,
pagine: 2001 - 2009
SICI:
1567-5769(200110)1:11<2001:AOMKOD>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MORPHINE-INDUCED ALTERATIONS; OPIOID RECEPTOR; IMMUNE STATUS; IN-VITRO; TOLERANCE DEVELOPMENT; PERIAQUEDUCTAL GRAY; LYMPHOCYTE ACTIVITY; OPIATE RECEPTORS; BINDING-SITES; INVOLVEMENT;
Keywords:
morphine; kappa opioid; delta(2) opioid; osmotic minipumps; immunosuppression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Eisenstein, TK Temple Univ, Sch Med, Dept Microbiol & Immunol, 3400 N Broad St, Philadelphia, PA 19140 USA Temple Univ 3400 N Broad St Philadelphia PA USA 19140 0 USA
Citazione:
R.T. Rahim et al., "Administration of mu-, kappa- or delta(2)-receptor agonists via osmotic minipumps suppresses murine splenic antibody responses", INT IMMUNO, 1(11), 2001, pp. 2001-2009

Abstract

Previously, our laboratory has shown that morphine given by implantation of a 75-mg slow-release pellet for 48 h suppresses murine splenic antibody responses to sheep red blood cells (SRBCs) in a plaque-forming cell (PFC) assay. However, the use of slow-release pellets for such studies is limited, as these pellets are only available in fixed doses and similar pellets for kappa and delta agonists have not been developed. In the present study, we investigated the feasibility of administering opioids via Alzet (R) osmoticminipumps to assess their immunomodulatory effects. Groups of mice received minipumps dispensing morphine sulfate, which has primary activity at the mu opioid receptor; U50,488H, which is a kappa-selective agonist; deltorphin II, which is a delta(2)-selective agonist; or DPDPE, which has greater selectivity for delta(1) than delta(2) receptors. Morphine, U50,488H and deltorphin II were all immunosuppressive, with biphasic dose-response curves exhibiting maximal (approximately 50%) suppression of the PFC response at doses of 0.5 to 2 mg/kg/day 48 h after pump implantation. Further, immunosuppression by morphine sulfate, U50,488H or deltorphin II was blocked by simultaneous implantation of a minipump administering the opioid receptor-selective antagonists CTAP (1 mg/kg/day), nor-binaltorphimine (5 mg/kg/day), or naltriben (3 mg/kg/day), respectively. DPDPE was inactive at doses lower than10 mg/kg/day. We conclude that osmotic minipumps are a practical and useful way of administering opioids to study their effects on the immune system,and give further evidence that immunosuppression induced in vivo by opioidagonists is mediated not only via mu, but also via kappa and delta(2) opioid receptors. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:28:24