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Titolo:
Macrophage-oriented cytotoxic activity of novel triterpene saponins extracted from roots of Securidaca inappendiculata
Autore:
Yui, S; Ubukata, K; Hodono, K; Kitahara, M; Mimaki, Y; Kuroda, M; Sashida, Y; Yamazaki, M;
Indirizzi:
Teikyo Univ, Fac Pharmaceut Sci, Sagamiko, Kanagawa 1990195, Japan Teikyo Univ Sagamiko Kanagawa Japan 1990195 miko, Kanagawa 1990195, Japan Kaneka Corp, Res Inst, Takasago Res Labs, Takasago, Hyogo 6768688, Japan Kaneka Corp Takasago Hyogo Japan 6768688 , Takasago, Hyogo 6768688, Japan Tokyo Univ Pharm & Life Sci, Sch Pharm, Lab Medicinal Plant Sci, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci Tokyo Japan 1920392 ci, Tokyo 1920392, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 11, volume: 1, anno: 2001,
pagine: 1989 - 2000
SICI:
1567-5769(200110)1:11<1989:MCAONT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CELL-PROLIFERATION; DNA-SYNTHESIS; INDUCTION; APOPTOSIS; GROWTH; ACTIVATION; PROTEASE; RECEPTOR; INSITU;
Keywords:
macrophages; apoptosis; M-CSF; saponin compounds;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Yui, S Teikyo Univ, Fac Pharmaceut Sci, Sagamiko, Kanagawa 1990195, Japan Teikyo Univ Sagamiko Kanagawa Japan 1990195 anagawa 1990195, Japan
Citazione:
S. Yui et al., "Macrophage-oriented cytotoxic activity of novel triterpene saponins extracted from roots of Securidaca inappendiculata", INT IMMUNO, 1(11), 2001, pp. 1989-2000

Abstract

It is recognized that macrophages in peripheral tissues often proliferate under pathological conditions such as tumors, inflammation and atherosclerosis. Because the growth state of macrophages is believed to be a factor regulating the pathological process of the diseases, substances that regulate macrophage growth or,survival may be useful for disease control. In this paper, we identified the activity inhibiting macrophage growth in a hot waterextract of roots of Securidaca inappendiculata. The extract markedly inhibited macrophage colony-stimulating factor (M-CSF/CSF-1)-induced growth of macrophages, whereas it exerted a less potent effect on growth of Concanavalin A (Con A)-stimulated thymocytes or M-CSF-stimulated bone marrow cells. The inhibition of macrophage growth was caused by a cytotoxic effect rather than a cytostatic effect. Cell death was due to the induction of apoptosis,as judged by staining with terminal deoxynucleotidyl transferase-mediated d-UTP nick end labelling (TUNEL). The cytotoxic activity seemed to be specific to peripheral macrophages; it showed a weak effect on the growth and survival of tumor cell lines including a macrophage-like cell line, J-774.1. Moreover, the saponin fraction induced apoptotic cell death of macrophages only when they were stimulated by M-CSF; it did not affect the viability ofmacrophages cultured without M-CSF or with granulocyte/macrophage-CSF. We determined the structures of the two active triterpene saponin compounds inthe fraction, named securioside A and securioside B having a 3,4-dimethoxycinnamic group which is essential for the cell death-inducing activity. They are believed to be the primary compounds of new drugs for the treatment of pathological states in which macrophage proliferation occurs. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:42:53