Catalogo Articoli (Spogli Riviste)

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Titolo:
Regulation of IL-13 production by histamine in cloned murine T helper type2 cells
Autore:
Elliott, KA; Osna, NA; Scofield, MA; Khan, MM;
Indirizzi:
Creighton Univ, Dept Pharmaceut & Adm Sci, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 armaceut & Adm Sci, Omaha, NE 68178 USA Creighton Univ, Dept Pharmacol, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 iv, Dept Pharmacol, Omaha, NE 68178 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 11, volume: 1, anno: 2001,
pagine: 1923 - 1937
SICI:
1567-5769(200110)1:11<1923:ROIPBH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-A; ANTIGEN-INDUCED IL-4; SIGNAL-TRANSDUCTION; INTERFERON-GAMMA; DIFFERENTIAL REGULATION; ELEVATING AGENTS; GENETIC-VARIANTS; HUMAN BASOPHILS; MESSENGER-RNA; TH2 CELLS;
Keywords:
histamine; interleukin-13; protein kinase A; Th2 lymphocytes; H-8; Rp-cAMPS; tyrphostin AG490; STAT6; interleukin-12; Janus kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Khan, MM Creighton Univ, Dept Pharmaceut & Adm Sci, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 & Adm Sci, Omaha, NE 68178 USA
Citazione:
K.A. Elliott et al., "Regulation of IL-13 production by histamine in cloned murine T helper type2 cells", INT IMMUNO, 1(11), 2001, pp. 1923-1937

Abstract

Histamine affects the balance of T helper type I (Th1) and T helper type 2(Th2) cytokines by shifting cytokine production from a Th1 to a Th2 pattern. Interleukin-13 (IL-13) is an important autacoid mediator that has been implicated in the development of allergic disease. This study was designed to investigate the mechanisms of regulation of IL-13 by histamine in Th2 cells. D10.G4.1 cells, a murine Th2 cell line, were treated with histamine (10(-8)-10(-4) M) and then activated with PMA (phorbol 12 myristate 13-acetate) plus ionomycin or alpha CD3. Levels of IL-13 production were then measured by enzyme-linked immunosorbent assay (ELISA) and semiquantitative reversetranscription-polymerase chain reaction (RT-PCR). Cells were pretreated with histamine receptor antagonists pyrilamine, ranitidine, cimetidine and thioperamide to determine the involvement of histamine receptors. Cells were also pretreated with protein kinase A (PKA) inhibitors N-[2-(methylaminoethyl)]-5-isoquinoline-sulfonamide (H-8) and Rp-diastereomer of adenosine cyclic 3 '5 ' -phosphorothionate (Rp-cAMPS), and Janus kinase-signal transducerand activator of transcription (Jak-STAT) inhibitor tyrphostin AG490 priorto the addition of histamine. H-8 is an inhibitor of the catalytic subunitof PKA while Rp-cAMPS is an inhibitor of the regulatory subunit of PKA. Tyrphostin is an inhibitor of Jak2, Jak3, STAT1, STAT3 and STAT5. Finally, cells were pretreated with IL-12, a monokine known to repress STAT6 DNA binding. We found that histamine dose-dependently enhanced IL-13 secretion and mRNA levels in Th2 cells via HI and H2 receptors. Pretreatment of cells withH-8, Rp-cAMPS and tyrphostin prevented histamine-induced secretion and transcription of IL-13. Likewise, pretreatment of Th2 cells with IL-12 also reversed histamine's effects on IL-13 secretion from stimulatory to inhibitory. These observations suggest a role for PKA and the Jak-STAT pathway in histamine-mediated elevation of IL-13 secretion and transcription. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/10/20 alle ore 10:23:08