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Titolo:
Systematic determination of the packaging limit of lentiviral vectors
Autore:
Kumar, M; Keller, B; Makalou, N; Sutton, RE;
Indirizzi:
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 l & Microbiol, Houston, TX 77030 USA Baylor Coll Med, Dept Med, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Med, Dept Med, Houston, TX 77030 USA Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Gene Therapy, Houston, TX 77030 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 15, volume: 12, anno: 2001,
pagine: 1893 - 1905
SICI:
1043-0342(20011015)12:15<1893:SDOTPL>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC STEM-CELLS; EFFICIENCY GENE-TRANSFER; NUCLEAR-LOCALIZATION; NONDIVIDING CELLS; TYPE-1 VECTORS; LEUKEMIA-VIRUS; PROTEIN; HIV-1; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Sutton, RE Baylor Coll Med, Dept Mol Virol & Microbiol, 1 Baylor Plaza,Rm 917D, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza,Rm 917D Houston TX USA 77030 USA
Citazione:
M. Kumar et al., "Systematic determination of the packaging limit of lentiviral vectors", HUM GENE TH, 12(15), 2001, pp. 1893-1905

Abstract

Because of their ability to transduce nondividing cells, human immunodeficiency virus type 1 (HIV)-based vectors have great potential for the therapeutic delivery of genes to cells. We describe here a systematic study of thepackaging limit of HIV-based vectors. Restriction endonuclease-generated bacterial chromosomal DNA fragments of different lengths were cloned at three different positions within a lentiviral vector. Vesicular stomatitis virus G protein (VSV G) pseudotyped lentiviral particles were prepared and the different clones were titered on mammalian cells. We observed that the restriction endonuclease site positions at the 5' and 3' ends of the genome were superior with regard to insertional capacity of foreign DNA. In all cases, viral titers decreased semi-logarithmically with increasing vector length. There appears to be no absolute packaging limit because measurable titerswere obtained even when the proviral length was in excess of 18 kb. The reduction in titer appears to occur at the level of viral encapsidation, although we cannot exclude limitations in nuclear export of proviral RNA. Theseresults suggest that HIV-based vectors may have a secondary advantage overoncoretroviral vectors because of their greater packaging limit, although the very low titers of the larger vectors will be of limited utility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:40:43