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Titolo:
Reduced albumin reabsorption in the proximal tubule of early-stage diabetic rats
Autore:
Tojo, A; Onozato, ML; Ha, HJ; Kurihara, H; Sakai, T; Goto, A; Fujita, T; Endou, H;
Indirizzi:
Univ Tokyo, Dept Internal Med, Div Nephrol & Endocrinol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 docrinol, Bunkyo Ku, Tokyo 1138655, Japan Soon Chun Hyang Univ, Hyonam Kidney Lab, Seoul, South Korea Soon Chun Hyang Univ Seoul South Korea m Kidney Lab, Seoul, South Korea Juntendo Univ, Dept Anat, Tokyo, Japan Juntendo Univ Tokyo JapanJuntendo Univ, Dept Anat, Tokyo, Japan Kyorin Univ, Dept Pharmacol & Toxicol, Tokyo, Japan Kyorin Univ Tokyo Japan in Univ, Dept Pharmacol & Toxicol, Tokyo, Japan
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 116, anno: 2001,
pagine: 269 - 276
SICI:
0948-6143(200109)116:3<269:RARITP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR-BASEMENT-MEMBRANE; NITRIC-OXIDE SYNTHASE; MITOCHONDRIAL ENLARGEMENT; URINARY-EXCRETION; RENAL HYPERTROPHY; MICROALBUMINURIA; PROTEINS; KIDNEY; PERMEABILITY; DEGRADATION;
Keywords:
diabetic nephropathy; microalbuminuria; proximal tubule; megalin; endocytosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Tojo, A Univ Tokyo, Dept Internal Med, Div Nephrol & Endocrinol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 , Tokyo 1138655, Japan
Citazione:
A. Tojo et al., "Reduced albumin reabsorption in the proximal tubule of early-stage diabetic rats", HISTOCHEM C, 116(3), 2001, pp. 269-276

Abstract

The aim of this study is to investigate the role of the proximal tubule inmicroalbuminuria in the early stage of diabetic nephropathy. Diabetes was induced in male Sprague-Dawley rats by an injection of streptozotocin (50 mg/kg, i.v.). After 2 weeks, albumin delivery in the proximal tubule was measured using micropuncture and the endocytosis process of FITC-labeled albumin was evaluated with immunoelectron microscopy. Albumin was significantly reabsorbed in the proximal convoluted tubule (PCT) of controls (0.39 +/-0.05 ng/min at early PCT to 0.17 +/-0.08 at late PCT, P <0.05), whereas albumin reabsorption was inhibited in diabetic rats (0.27 +/-0.05 to 0.21 +/-0.08). Immunogold study revealed that FITC-albumin was significantly less reabsorbed in endosomes and lysosomes of S1 segments in diabetic rats than in controls (endosome: 1.20 +/-0.10 vs 2.16 +/-0.15 mum(-1) P <0.0001; lysosome:0.26 +/-0.03 vs 0.83 +/-0.07, P <0.0001). The expression of megalin, an endocytosis receptor, was decreased at the apical membrane of PCT in diabeticrats. The lipid peroxidation production in the proximal tubule was significantly increased in diabetic rats. In conclusion, albuminuria in early-stage diabetic rats can be partly explained by a decreased albumin endocytosis with reduced megalin expression and with increased lipid peroxidation in the proximal tubule.

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Documento generato il 31/03/20 alle ore 22:40:58