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Titolo:
Evaluation of the total number of myenteric neurons in the developing chicken gut using cuprolinic blue histochemical staining and neurofilament immunocytochemistry
Autore:
Roman, V; Krecsmarik, M; Bagyanszki, M; Fekete, E;
Indirizzi:
Univ Szeged, Dept Zool & Cell Biol, H-6722 Szeged, Hungary Univ Szeged Szeged Hungary H-6722 ol & Cell Biol, H-6722 Szeged, Hungary
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 116, anno: 2001,
pagine: 241 - 246
SICI:
0948-6143(200109)116:3<241:EOTTNO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTERIC NERVOUS-SYSTEM; PIG SMALL-INTESTINE; GUINEA-PIG; POSTNATAL-DEVELOPMENT; COUNT NEURONS; PLEXUS; RAT; GANGLIA;
Keywords:
chicken gut; development; cuprolinic blue; neurofilament; quantitative study;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Fekete, E Univ Szeged, Dept Zool & Cell Biol, Egyetem U 2,POB 659, H-6722 Szeged, Hungary Univ Szeged Egyetem U 2,POB 659 Szeged Hungary H-6722 , Hungary
Citazione:
V. Roman et al., "Evaluation of the total number of myenteric neurons in the developing chicken gut using cuprolinic blue histochemical staining and neurofilament immunocytochemistry", HISTOCHEM C, 116(3), 2001, pp. 241-246

Abstract

The aim of this study was to find an improved method with which to stain the entire population of myenteric neurons in the different segments of the developing chicken intestine. Histochemical staining with cuprolinic blue (quinolinic phthalocyanine) and immunostaining against neurofilament (NF) were performed on whole mounts prepared from intestinal segments of embryonic(day 19 of incubation) and hatched (1, 2, 4 and 7 days after hatching) chickens. Double labelling was performed to evaluate to what extent the two markers visualise the same nerve cell population. Cuprolinic blue stained neuronal somata highly selectively, whereas processes and glia cells were poorly labelled. The cuprolinic blue-positive neurons were uniform in shape. NFimmunostaining revealed a morphologically highly variable neuron population. Double labelling with cuprolinic blue and NF resulted in an intensification of both stainings, allowing an accurate morphological classification ofNF-stained myenteric neurons. Data obtained from the counting of cuprolinic blue-positive neurons were subjected to two-way ANOVA and the Tukey probe. The densities of ganglia and neurons were found,to decrease, and the meannumber of neurons per myenteric ganglion to increase, with different dynamics along the longitudinal axis of the gut during the examined time span. The variances in the number of NF-positive neurons were not homogeneous, andthe data were therefore not suitable for ANOVA. Accordingly, only semiquantitative conclusions could be drawn.

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Documento generato il 25/11/20 alle ore 15:43:31