Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Opioid system diversity in developing neurons, astroglia, and oligodendroglia in the subventricular zone and striatum: Impact on gliogenesis in vivo
Autore:
Stiene-Martin, A; Knapp, PE; Martin, K; Gurwell, JA; Ryan, S; Thornton, SR; Smith, FL; Hauser, KF;
Indirizzi:
Univ Kentucky, Coll Med, Dept Anat & Neurobiol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 & Neurobiol, Lexington, KY 40536 USA Virginia Commonwealth Univ, Med Coll Virginia, Dept Pharmacol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ Med Coll Virginia, Dept Toxicol, Richmond, VA USA Virginia Commonwealth Univ Med Coll Virginia Richmond VA USA ond, VA USA
Titolo Testata:
GLIA
fascicolo: 1, volume: 36, anno: 2001,
pagine: 78 - 88
SICI:
0894-1491(200110)36:1<78:OSDIDN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; CENTRAL-NERVOUS-SYSTEM; C6 GLIOMA-CELLS; DIFFERENTIAL POSTNATAL-DEVELOPMENT; KAPPA-OPIATE RECEPTORS; PROTEIN-KINASE-C; RAT-BRAIN; DNA-SYNTHESIS; CA2+ MOBILIZATION; IN-VITRO;
Keywords:
astrocytes; oligodendrocytes; cell division; subventricular zone; striatum; mu-opioid receptors; delta-opioid receptors; kappa-opioid receptors; central nervous system development; opiate drug abuse; glial fibrillary acidic protein; S100 beta; drug abuse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Hauser, KF Univ Kentucky, Coll Med, Dept Anat & Neurobiol, 800 Rose St, Lexington, KY40536 USA Univ Kentucky 800 Rose St Lexington KY USA 40536 n, KY40536 USA
Citazione:
A. Stiene-Martin et al., "Opioid system diversity in developing neurons, astroglia, and oligodendroglia in the subventricular zone and striatum: Impact on gliogenesis in vivo", GLIA, 36(1), 2001, pp. 78-88

Abstract

Accumulating evidence, obtained largely in vitro, indicates that opioids regulate the genesis of neurons and glia and their precursors in the nervoussystem. Despite this evidence, few studies have assessed opioid receptor expression in identified cells within germinal zones or examined opioid effects on gliogenesis in vivo. To address this question, the role of opioids was explored in the subventricular zone (SVZ) and/or striatum of 2-5-day-oldand/or adult ICR mice. The results showed that subpopulations of neurons, astrocytes, and oligodendrocytes in the SVZ and striatum differentially express mu-, delta-, and/or kappa -receptor immunoreactivity in a cell type-specific and developmentally regulated manner. In addition, DNA synthesis wasassessed by examining 5-bromo-2'-deoxyuridine (BrdU) incorporation into glial and nonglial precursors. Morphine (a preferential mu -agonist) significantly decreased the number of BrdU-labeled GFAP(+) cells compared with controls or mice co-treated with naltrexone plus morphine. Alternatively, in S100 beta (+) cells, morphine did not significantly decrease BrdU incorporation; however, significant differences were noted between mice treated with morphine and those treated with morphine plus naltrexone. Most cells were GFAP(-)/S100 beta (-). When BrdU incorporation was assessed within the total population (glia and nonglia), morphine had no net effect, but naltrexone alone markedly increased BrdU incorporation. This finding suggests that DNA synthesis in GFAP(-)/S100 beta (-) cells is tonically suppressed by endogenous opioids. Assuming that S100 beta and GFAP, respectively, distinguish among younger and older astroglia, this implies that astroglial replication becomes increasingly sensitive to morphine during maturation, and suggests that opioids differentially regulate the development of distinct subpopulations of glia and glial precursors. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:27:29