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Titolo:
Vascular endothelial growth factor induces manganese-superoxide dismutase expression in endothelial cells by a Rac1-regulated NADPH oxidase-dependentmechanism
Autore:
Abid, MR; Tsai, JC; Spokes, KC; Deshpande, SS; Irani, K; Aird, WC;
Indirizzi:
Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 d, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr, Dept Mol Med, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 d, Boston, MA 02215 USA Harvard Univ, Sch Med, Boston, MA USA Harvard Univ Boston MA USAHarvard Univ, Sch Med, Boston, MA USA Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 pt Med, Baltimore, MD 21205 USA
Titolo Testata:
FASEB JOURNAL
fascicolo: 11, volume: 15, anno: 2001,
pagine: NIL_211 - NIL_232
SICI:
0892-6638(200109)15:11<NIL_211:VEGFIM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; NITRIC-OXIDE; ANGIOTENSIN-II; SIGNAL-TRANSDUCTION; IN-VIVO; CRITICAL COMPONENT; OXIDATIVE STRESS; NAD(P)H OXIDASE;
Keywords:
gene regulation; cell signaling; endothelial cell migration; reactive oxygen species; vascular endothelial growth factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Aird, WC Beth Israel Deaconess Med Ctr, Dept Med, RW-663,330 Brookline Ave, Boston,MA 02215 USA Beth Israel Deaconess Med Ctr RW-663,330 Brookline Ave Boston MA USA 02215
Citazione:
M.R. Abid et al., "Vascular endothelial growth factor induces manganese-superoxide dismutase expression in endothelial cells by a Rac1-regulated NADPH oxidase-dependentmechanism", FASEB J, 15(11), 2001, pp. NIL_211-NIL_232

Abstract

Vascular endothelial growth factor (VEGF) is a potent vascular endothelialcell-specific mitogen that modulates endothelial cell function. In the present study, we show that VEGF induces manganese-superoxide dismutase (MnSOD) mRNA and protein in human coronary artery endothelial cells (HCAEC) and pulmonary artery endothelial cells. VEGF-mediated induction of MnSOD mRNA was inhibited by pretreatment with the NADPH oxidase inhibitors, diphenyleneiodonium (DPI), and 4-(2-aminoethyl)-benzenesulfonyl fluoride, but not with the nitric oxide synthase inhibitor L-NAME (N-monomethyl-L-arginine) or thexanthine oxidase inhibitor allopurinol. VEGF stimulation of MnSOD was alsoinhibited by adenoviral-mediated overexpression of catalase Cu, Zn-SOD anda dominant-negative form of the small GTPase component of NADPH oxidase Rac1 (Rac1N17). Treatment of HCAEC with VEGF resulted in a transient increasein ROS production at 20 min, as measured by 2',7'-dichlorodihydrofluorescein oxidation. This effect was abrogated by expression of Rac1N17. Taken together, these findings suggest that VEGF induces MnSOD by an NADPH oxidase-dependent mechanism and that VEGF signaling in the endothelium is coupled tothe redox state of the cell.

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Documento generato il 18/09/20 alle ore 15:57:01