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Titolo:
Apical to basolateral transcytosis and apical recycling of immunoglobulin G in trophoblast-derived BeWo cells: Effects of low temperature, nocodazole, and cytochalasin D
Autore:
Ellinger, I; Rothe, A; Grill, M; Fuchs, R;
Indirizzi:
Univ Vienna, Dept Pathophysiol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 t Pathophysiol, A-1090 Vienna, Austria
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 269, anno: 2001,
pagine: 322 - 331
SICI:
0014-4827(20011001)269:2<322:ATBTAA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEONATAL FC RECEPTOR; CANINE KIDNEY-CELLS; POLARIZED EPITHELIAL-CELLS; I-RELATED RECEPTOR; MDCK CELLS; MEDIATED ENDOCYTOSIS; ACTIN-FILAMENTS; BIDIRECTIONAL TRANSCYTOSIS; MAMMALIAN-CELLS; HUMAN PLACENTA;
Keywords:
IgG-transport; trophoblasts; choriocarcinoma cells; hFcRn; actin; microtubules; temperature;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, R Univ Vienna, Dept Pathophysiol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 Austria
Citazione:
I. Ellinger et al., "Apical to basolateral transcytosis and apical recycling of immunoglobulin G in trophoblast-derived BeWo cells: Effects of low temperature, nocodazole, and cytochalasin D", EXP CELL RE, 269(2), 2001, pp. 322-331

Abstract

The murine neonatal Fc receptor, FcRn, carries out two functions: materno-fetal IgG delivery and maintenance of serum IgG homeostasis. During human pregnancy maternal IgG is transferred across placental syncytiotrophoblasts presumably by the human homolog of FcRn, hFcRn. Trophoblast-derived BeWo cells express hFcRn endogenously and can be considered as a model system to investigate IgG transport in syncytiotrophoblasts. Using a pulse-chase protocol, we here demonstrate that polarized BeWo cells exhibit not only apical to basolateral transcytosis but also apical IgG recycling. Thus, for the first time we demonstrate that epithelial cells can be involved in both materno-fetal IgG transmission and regulation of serum IgG levels. Lowering the temperature from 37 to 16 degreesC reduced, but did not block, IgG recycling and transcytosis. Microtubule-disruption by nocodazole did not influence transcytosis or apical recycling. Disassembly of filamentous actin by cytochalasin D stimulated apical endocytosis and recycling, while transcytosis remained unaffected. In summary, in BeWo cells apically internalized IgG enters both a transcytotic and recycling pathway. While the transcytotic routeis temperature-sensitive but independent from microtubules and actin filaments, the apical recycling pathway is temperature-influenced and stimulatedby actin disassembly, suggestive for the involvement of distinct endosome subcompartments in transcytosis and recycling. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:35:27