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Titolo:
MyoD activity upregulates E2F1 and enhances transcription from the cyclin E promoter in differentiating myoblasts lacking a functional retinoblastomaprotein
Autore:
Tedesco, D; Vesco, C;
Indirizzi:
CNR, Ist Biol Cellulare, I-00137 Rome, Italy CNR Rome Italy I-00137CNR, Ist Biol Cellulare, I-00137 Rome, Italy
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 269, anno: 2001,
pagine: 301 - 311
SICI:
0014-4827(20011001)269:2<301:MAUEAE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE T-ANTIGEN; LOOP-HELIX PROTEINS; CELL-CYCLE; MYOGENIC DIFFERENTIATION; GENE-EXPRESSION; DNA-BINDING; HISTONE DEACETYLASE; FAMILY PROTEINS; PROLIFERATING MYOBLASTS; REPRESS TRANSCRIPTION;
Keywords:
MyoD; E2F1; Rb protein; cyclin E; myoblasts; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Vesco, C CNR, Ist Biol Cellulare, Vle Marx 43, I-00137 Rome, Italy CNR VleMarx 43 Rome Italy I-00137 Marx 43, I-00137 Rome, Italy
Citazione:
D. Tedesco e C. Vesco, "MyoD activity upregulates E2F1 and enhances transcription from the cyclin E promoter in differentiating myoblasts lacking a functional retinoblastomaprotein", EXP CELL RE, 269(2), 2001, pp. 301-311

Abstract

We investigated the mechanism leading to cyclin E accumulation when cultured mouse myoblasts, lacking functional Rb because of sequestration or deletion, are exposed to differentiating conditions (mitogen subtraction and cell-cell contact), which activate MyoD and normally downregulate factors involved in cell division. After excluding that stabilization might account forthe observed cyclin-E mRNA accumulation, we found an induction of the cyclin-E promoter that correlated with E2F activity upregulation and depended on both MyoD activation and Rb inactivation. Analyses of the E2F1-promoter activity, in normal and Rb-deficient fibroblasts converted by MyoD, identified a MyoD function stimulating E2F1 expression. The E2F1 induction was verymanifest in the Rb-/- cells, but also detectable, at the early stage of differentiation, in normal cells. Its effects, although not indispensable formyogenesis, presumably contribute to raise the concentration of Rb-E2F1 transcription-repressing complexes, since MyoD strongly induces also Rb in differentiating myocytes. The activity of an E2F1 promoter lacking the E2F sites indicated that E2F1 itself underwent self-repression by such mechanism at late stages of differentiation. In the absence of Rb, however, the induced E2F1 is left with only its activating role, reversing the normal effect of this MyoD function. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 00:19:54