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Titolo:
Experimental designs to investigate capillary electrophoresis-electrosprayionization-mass spectrometry enantioseparation with the partial-filling technique
Autore:
Rudaz, S; Cherkaoui, S; Gauvrit, JY; Lanteri, P; Veuthey, JL;
Indirizzi:
Univ Geneva, Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 lyt Chem, CH-1211 Geneva 4, Switzerland Univ Lyon 1, ESPCE Lyon, Lab Chemometrics, Villeurbanne, France Univ Lyon 1 Villeurbanne France Lab Chemometrics, Villeurbanne, France
Titolo Testata:
ELECTROPHORESIS
fascicolo: 15, volume: 22, anno: 2001,
pagine: 3316 - 3326
SICI:
0173-0835(200109)22:15<3316:EDTICE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFINITY ELECTROKINETIC CHROMATOGRAPHY; LOCAL-ANESTHETIC DRUGS; CHIRAL SEPARATION; ZONE ELECTROPHORESIS; THEORETICAL ASPECTS; ALPHA(1)-ACID GLYCOPROTEIN; BETA-BLOCKERS; ONLINE; ENANTIOMERS; SELECTORS;
Keywords:
enantioseparation; cyclodextrins; capillary electrophoresis; mass spectrometry; partial-filling technique; chemometrics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Veuthey, JL Univ Geneva, Lab Pharmaceut Analyt Chem, 20 Bd Yvoy, CH-1211 Geneva 4, Switzerland Univ Geneva 20 Bd Yvoy Geneva Switzerland 4 va 4, Switzerland
Citazione:
S. Rudaz et al., "Experimental designs to investigate capillary electrophoresis-electrosprayionization-mass spectrometry enantioseparation with the partial-filling technique", ELECTROPHOR, 22(15), 2001, pp. 3316-3326

Abstract

An experimental design approach is described to evaluate the main electrophoretic parameters involved in the enantioseparation of pharmaceuticals by capillary electrophoresis (CE) coupled to electrospray ionization-mass spectrometry (ESI-MS). For all experiments, the partial-filling technique was applied to avoid the chiral selector entering in the mass spectrometer ion source with a negative effect on the electrospray performance. To carry out enantioseparation, a volatile buffer constituted of 20 mm ammonium acetate at pH 4.0, and a polyvinyl alcohol-coated capillary were used. Methadone was employed as the model compound and three different cyclodextrins (CDs), namely sulfobutyl ether-beta -CD, carboxymethylated-beta -CD and hydroxypropyl-beta -CD, were selected in order to study the countercurrent process. Two different experimental designs were chosen: (i) a full-factorial design to examine the effects and significance of the investigated factors, and (ii) a central composite face-centered design to establish the mathematical model of the selected responses in function of experimental factors. The chiral selector concentration, percentage of the capillary filled with the chiral selector, and drying gas nebulization pressure were three relevant factors taken into consideration. For each CID, the methadone enantiomeric resolution, apparent selectivity, and migration time of the second enantiomer were established as responses. The latter were systematically related to experimental parameters with the help of multiple linear regression. It is noteworthy that the behaviour was different in function of the chiral selector charge. Results revealed that the nebulization pressure involved in the electrospray process and the CID concentration had a significant effect on theenantiomeric resolution, while the effect of the separation zone length was less pronounced. Finally, response surfaces were drawn from the mathematical model and experimental conditions were selected to allow a robust determination of methadone enantiomers by CE-MS.

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Documento generato il 03/04/20 alle ore 20:07:11