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Titolo:
Absence of SV-40 large T antigen (Tag) in malignant mesothelioma effusions: An immunocytochemical study
Autore:
Simsir, A; Fetsch, P; Bedrossian, CWM; Ioffe, OB; Abati, A;
Indirizzi:
NYU, Med Ctr, Dept Pathol, New York, NY 10016 USA NYU New York NY USA 10016 U, Med Ctr, Dept Pathol, New York, NY 10016 USA
Titolo Testata:
DIAGNOSTIC CYTOPATHOLOGY
fascicolo: 4, volume: 25, anno: 2001,
pagine: 203 - 207
SICI:
8755-1039(200110)25:4<203:AOSLTA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PLEURAL MESOTHELIOMA; DNA-SEQUENCES; SIMIAN-VIRUS-40; ASBESTOS; DISEASES; TISSUE; GENE;
Keywords:
simian virus 40; large T antigen; pleural effusion; mesothelioma; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Simsir, A NYU, Med Ctr, Dept Pathol, 530 1st Ave,Suite 7S,West Tower, New York, NY 10016 USA NYU 530 1st Ave,Suite 7S,West Tower New York NY USA 10016 16 USA
Citazione:
A. Simsir et al., "Absence of SV-40 large T antigen (Tag) in malignant mesothelioma effusions: An immunocytochemical study", DIAGN CYTOP, 25(4), 2001, pp. 203-207

Abstract

Simian Virus 40 (SV 40) was recently implicated in the pathogenesis of malignant mesothelioma. The oncogenic capacity of SV-40 is a function of a nuclear protein, the large T antigen (Tag). SV-40 Tag DNA sequences are detected by the polymerase chain reaction in 40-80% of malignant mesothelial proliferations. However, the role of immunohistochemistry (IHC) in demonstrating the nuclear localization of Tag is controversial. We sought to determine the clinical utility of SV-40 Tag IHC in pleural effusion cytology as an ancillary tool in the cytologic diagnosis of malignant mesothelioma (MM). Formalin-fixed, paraffin-embedded cell block sections from 100 pleural effusions (32 MMs, 25 benign reactive, 43 metastatic adenocarcinomas) were immunostained for the SV-40 anti-Tag, using two primary monoclonal SV-40 Tag antibodies: clone Pab 416 and clone Pab 101. Despite strong and consistent immunoreactivity in positive controls, no nuclear immunostaining was observed inan), case. We believe the small sample size in cytology cell block sections, the low viral copy number in infected cells, and the effect of formalin fixation may have resulted in absence of immunoreactivity. The role of SV-40 Tag IHC in diagnostic cytopathology remains unclear unless further studies reliably show its detection.(C) 2001 Wiley-Liss,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:28:58