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Titolo:
Msx2 is a repressor of chondrogenic differentiation in migratory cranial neural crest cells
Autore:
Takahashi, K; Nuckolls, GH; Takahashi, I; Nonaka, K; Nagata, M; Ikura, T; Slavkin, HC; Shum, L;
Indirizzi:
NIAMS, Craniofacial Dev Sect, NIH, Bethesda, MD 20892 USA NIAMS Bethesda MD USA 20892 ofacial Dev Sect, NIH, Bethesda, MD 20892 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 2, volume: 222, anno: 2001,
pagine: 252 - 262
SICI:
1058-8388(200110)222:2<252:MIAROC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAL DYE ANALYSIS; MOUSE EMBRYOS; TOOTH DEVELOPMENT; GENE-EXPRESSION; TRANSGENIC MICE; COLLAGEN GENE; II COLLAGEN; DNA-BINDING; IN-VITRO; SOX9;
Keywords:
cranial neural crest cell migration; cell fate determination; mandibulofacial dysostosis; mouse embryo; adenovirus gene delivery; loss-of-function; mutagenesis; Msx2; Sox9; Meckel's cartilage; alcian blue staining; type II collagen; aggrecan; explant culture; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Shum, L NIAMS, Craniofacial Dev Sect, NIH, Room 324,Bldg 6,6 Ctr Dr,MSC-2745, Bethesda, MD 20892 USA NIAMS Room 324,Bldg 6,6 Ctr Dr,MSC-2745 BethesdaMD USA 20892 USA
Citazione:
K. Takahashi et al., "Msx2 is a repressor of chondrogenic differentiation in migratory cranial neural crest cells", DEV DYNAM, 222(2), 2001, pp. 252-262

Abstract

During early mouse embryogenesis, cranial neural crest cells (CNCC) emigrate from the posterior midbrain and rhombomeres I and 2 of the anterior hindbrain into the first branchial arch-derived maxillary and mandibular processes and there provide cell lineages for several phenotypes, including cartilage, bone, and tooth. Here, we report that Sox9 and Msx2 were coexpressed in a subpopulation of CNCC during their migration. Because Sox9 is a transactivator of chondrogenesis, and Msx genes can act as transcriptional repressors, we hypothesized that Sox9 expression indicates the determination of CNCC-derived chondrogenic cell lineage and that Msx2. represses chondrogenicdifferentiation until CNCC migration is completed within the mandibular processes. To test whether Msx2 represses chondrogenesis,, we designed experiments to inhibit Msx2 function in migratory CNCC in primary cultures through the expression of loss-of-function Msx2 mutants. We showed that infectionof migratory CNCC with adenovirus Msx2 mutants accelerated the rate and extent of chondrogenesis, as indicated by the expression level of type II collagen and aggrecan, and the amount of alcian blue staining. Adenovirus infections did not apparently interfere with CNCC proliferation or migration. These findings suggest that an important early event in craniofacial morphogenesis is a transient expression of both Sox9 and Msx2 during emigration into the forming mandibular processes followed by restricted expression of Sox9 within CNCC-derived chondroprogenitor cells. We conclude that Msx2 serves as a repressor of chondrogenic differentiation during CNCC migration. Published 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:29:08