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Titolo:
Central role of the alpha 4 beta 1 integrin in the coordination of avian truncal neural crest cell adhesion, migration, and survival
Autore:
Testaz, S; Duband, JL;
Indirizzi:
Univ Paris 06, CNRS UMR 7622, Dev Biol Lab, F-75005 Paris, France Univ Paris 06 Paris France F-75005 , Dev Biol Lab, F-75005 Paris, France
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 2, volume: 222, anno: 2001,
pagine: 127 - 140
SICI:
1058-8388(200110)222:2<127:CROTA4>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; ANTISENSE OLIGONUCLEOTIDES; EXTRACELLULAR-MATRIX; ALPHA(4) INTEGRINS; CROSS-TALK; FIBRONECTIN; EXPRESSION; VLA-4; INHIBITION; DISTINCT;
Keywords:
avian embryo; neural crest; cell migration; cell adhesion; cell survival; integrins; alpha 4 beta 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Duband, JL Univ Paris 06, CNRS UMR 7622, Dev Biol Lab, 9 Quai St Bernard,7eme Etage, F-75005 Paris, France Univ Paris 06 9 Quai St Bernard,7eme EtageParis France F-75005
Citazione:
S. Testaz e J.L. Duband, "Central role of the alpha 4 beta 1 integrin in the coordination of avian truncal neural crest cell adhesion, migration, and survival", DEV DYNAM, 222(2), 2001, pp. 127-140

Abstract

Based on functional and histological studies, the fibronectin receptor of the integrin family alpha4 beta1 has been ascribed a critical role during neural crest cell migration in the vertebrate embryo. In the present study, because integrins have been shown to participate in multiple basic cellularprocesses, including cell adhesion, migration, survival, proliferation, and differentiation, we have reexamined in detail the role of alpha4 beta1 during avian truncal neural crest cell migration. RT-PCR and immunocytochemical studies revealed that migrating neural crest cells but not premigratory cells explanted in vitro expressed detectable levels of alpha4 messengers and proteins suggesting that alpha4 beta1 expression was induced at the timeof the initiation of the migration phase. In agreement with this observation, antibody inhibition of alpha4 beta1 activity in vitro resulted in a strong, immediate and sustained reduction of neural crest cell motion on fibronectin, as judged on videomicroscopy analyses, but apparently did not prevent their delamination from the neural tube. However, alpha4 beta1 appeared to exhibit a broader role in the control of cell migration on a variety of extracellular matrix molecules, presumably by regulating cellular events downstream from integrins. Moreover, blocking alpha4 beta1 function caused a severe increase in apoptotic cell death among the neural crest population without influencing notably cell proliferation. Collectively, these results indicate that, notwithstanding its critical implication in cell motion, alpha4 beta1 integrin could play a central role in neural crest cell development by coordinating multiple cellular events, such as cell adhesion, locomotion, and survival. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:02:40