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Titolo:
Development of rolipram-sensitive, cyclic AMP phosphodiesterase (PDE4) in rat primary neuronal cultures
Autore:
Ye, Y; Jackson, K; Houslay, MD; Chandler, LJ; ODonnell, JM;
Indirizzi:
Univ Tennessee, Hlth Sci Ctr, Dept Pharmacol, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 Dept Pharmacol, Memphis, TN 38163 USA Med Univ S Carolina, Dept Physiol & Neurosci, Charleston, SC 29425 USA MedUniv S Carolina Charleston SC USA 29425 sci, Charleston, SC 29425 USA Univ Tennessee, Ctr Hlth Sci, Dept Pharmacol, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 Dept Pharmacol, Memphis, TN 38163 USA
Titolo Testata:
DEVELOPMENTAL BRAIN RESEARCH
fascicolo: 1, volume: 130, anno: 2001,
pagine: 115 - 121
SICI:
0165-3806(20010923)130:1<115:DORCAP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAMP-SPECIFIC PHOSPHODIESTERASE; LOW-K-M; NUCLEOTIDE PHOSPHODIESTERASES; NORADRENERGIC LESIONS; STIMULATING-HORMONE; INDUCED IMPAIRMENTS; BRAIN; MEMORY; INHIBITORS; EXPRESSION;
Keywords:
phosphodiesterase; neuron; cyclic AMP; tetrodotoxin; synapsin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: O'Donnell, JM Univ Tennessee, Hlth Sci Ctr, Dept Pharmacol, 874 Union Ave,Memphis, TN 38163 USA Univ Tennessee 874 Union Ave Memphis TN USA 38163 38163 USA
Citazione:
Y. Ye et al., "Development of rolipram-sensitive, cyclic AMP phosphodiesterase (PDE4) in rat primary neuronal cultures", DEV BRAIN R, 130(1), 2001, pp. 115-121

Abstract

The development of PDE4 was examined in primary neuronal cultures of rat cerebral cortex. Three days after culturing, neurons exhibited relatively low PDE4 activity (i.e., rolipram-sensitive PDE activity). It gradually increased over time, approximately doubling by day 12. The increase in activity was accompanied by an increase in the expression of the PDE4A variants, PDE4A5 and PDE4A1, as well as of the synaptic marker protein synapsin I. Therewas a strong correlation between the expression of the PDE4A variants withthat of synapsin 1, which suggests that as neurons develop and signal transduction increases there is a regulated increase in PDE4 expression and activity. Consistent with this interpretation, it was found that treatment with the sodium channel blocker tetrodotoxin, which inhibits depolarization-induced neurotransmitter release, reduced the expression of the PDE4A variants. These data demonstrate the developmental regulation of PDE4 in neurons and offer a manner by which the association of PDE4 variants with particularsignal transduction pathways may be studied in vitro. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 02/12/20 alle ore 18:33:10